Argininosuccinate Synthase Antibody - BSA Free

Western Blot: Argininosuccinate Synthase Antibody [NBP1-31522] - A. 30 ug A431 whole cell lysate/extract 10 % SDS-PAGE Argininosuccinate Synthase antibody dilution: 1:1000

Immunohistochemistry-Paraffin: Argininosuccinate Synthase Antibody [NBP1-31522] - Cal27 xenograft, using ASS1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Argininosuccinate Synthase Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Argininosuccinate Synthase. The exact sequence is proprietary.
• Predicted Species: Mouse (97%), Rat (97%), Bovine (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ASS1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (82%), Xenopus laevis (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Argininosuccinate Synthase Antibody - BSA Free

• argininosuccinate synthase 1
• argininosuccinate synthase
• argininosuccinate synthetase 1
• argininosuccinate synthetase
• ASSCTLN1
• citrulline-aspartate ligase
• Citrulline--aspartate ligase
• EC 6.3.4.5

Background

The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ASS1