Adenylosuccinate Lyase Antibody (OTI2D10)

Images

 
Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as ...read more
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human lymphoma tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.
Flow Cytometry: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ...read more
Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adenylosuccinate Lyase (Right lane) cDNA for 48 hrs and ...read more
Western Blot: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Adenylosuccinate Lyase monoclonal antibody.
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Adenylosuccinate Lyase mouse monoclonal ...read more
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human Kidney tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Adenylosuccinate Lyase Antibody (OTI2D10) [NBP2-03107] - Staining of paraffin-embedded Human liver tissue using anti-Adenylosuccinate Lyase mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI2D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.55 mg/ml

Order Details

Adenylosuccinate Lyase Antibody (OTI2D10) Summary

Immunogen
Full length human recombinant protein of human ADSL (NP_000017) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ADSL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Application Notes
Use in ICC/IF reported in scientific publication (PMID: 32439803).
Theoretical MW
54.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-03107 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.55 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Adenylosuccinate Lyase Antibody (OTI2D10)

  • adenylosuccinase
  • adenylosuccinate lyase
  • AMPS
  • ASase
  • ASL
  • EC 4.3.2
  • EC 4.3.2.2

Background

Adenylsuccinate lyase is involved in both de novo synthesis of purines and formation of adenosine monophosphate from inosine monophosphate. It catalyzes two reactions in AMP biosynthesis: the removal of a fumarate from succinylaminoimidazole carboxamide (SAICA) ribotide to give aminoimidazole carboxamide ribotide (AICA) and removal of fumarate from adenylosuccinate to give AMP. Adenylosuccinase deficiency results in succinylpurinemic autism, psychomotor retardation, and , in some cases, growth retardation associated with muscle wasting and epilepsy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Adenylosuccinate Lyase Antibody (NBP2-03107)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ADSL
Entrez