ARG2 Antibody - BSA Free

Western Blot: ARG2 Antibody [NBP2-15445] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ARG2 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: ARG2 Antibody [NBP2-15445] - Paraffin-embedded mouse kidney. ARG2 antibody dilution: 1:500.

Western Blot: ARG2 Antibody [NBP2-15445] - 293T whole cell and cytoplasm extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ARG2 antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: ARG2 Antibody [NBP2-15445] - Immunohistochemical analysis of paraffin-embedded U373 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: ARG2 Antibody [NBP2-15445] - Paraffin-embedded mouse duodenum. ARG2 antibody dilution: 1:500.

ARG2 antibody detects ARG2 protein at mitochondria by immunofluorescent analysis.Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.Green: ARG2 protein stained by ARG2 antibody (NBP2-15445) diluted at 1:500.Blue: Hoechst 33342 staining.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ARG2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ARG2 Antibody - BSA Free (NBP2-15445) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ARG2. The exact sequence is proprietary.
• Localization: Mitochondrion
• Predicted Species: Rhesus Macaque (98%), Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARG2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Dog (83%), Rabbit (88%), Chicken (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ARG2 Antibody - BSA Free

• ARG2
• Arginase 2
• arginase, type II
• Arginase-2
• arginase-2, mitochondrial
• EC 3.5.3
• EC 3.5.3.1
• kidney arginase
• Kidney-type arginase
• L-arginine amidinohydrolase
• L-arginine ureahydrolase
• nonhepatic arginase
• Non-hepatic arginase
• Type II Arginase

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ARG2
• Uniprot:
  • P78540()