OAT Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: OAT Antibody [NBP1-83239] - Analysis in human duodenum and pancreas tissues. Corresponding OAT RNA-seq data are presented for the same tissues.
Western Blot: OAT Antibody [NBP1-83239] - Analysis in control (vector only transfected HEK293T lysate) and OAT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: OAT Antibody [NBP1-83239] - Staining of human duodenum shows strong granular cytoplasmic postivity in glandular cells.
Immunohistochemistry-Paraffin: OAT Antibody [NBP1-83239] - Staining of human cerebral cortex shows moderate to strong granular cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: OAT Antibody [NBP1-83239] - Staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.
Immunohistochemistry-Paraffin: OAT Antibody [NBP1-83239] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Product Details

Summary
Reactivity Hu, Bv, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

OAT Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ALKSQVDKLTLTSRAFYNNVLGEYEEYITKLFNYHKVLPMNTGVEAGETACKLARKWGYTVKGIQKYKAKIVFAAGNFWGRTLSAISSSTDPTSYDGFGPFMPGFDIIPYNDLPALERALQDPNVAAFMVEPIQGEAGV
Predicted Species
Mouse (96%), Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OAT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04 - 0.4 ug/mL
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
OAT Recombinant Protein Antigen (NBP1-83239PEP)
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-83239 in the following applications:

Reactivity Notes

Bovine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for OAT Antibody

  • GACR
  • HOGA
  • OAT ornithine aminotransferase
  • OATASE
  • OKT

Background

OAT, also known as mitochondrial enzyme ornithine aminotransferase, is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. ( L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid.) Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Recombinant OAT protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OAT Antibody (NBP1-83239) (0)

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Reviews for OAT Antibody (NBP1-83239) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Bovine.

Reviews using NBP1-83239:
Filter by Applications
WB
(1)
Blocking test with OAT Recombinant Protein Antigen.
(1)
All Applications
Filter by Species
Bovine
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot OAT NBP1-83239
Enlarge
5
reviewed by:
Verified Customer
WB Bovine 02/01/2019
View

Summary

ApplicationWestern Blot
Sample Testedcow liver tissue
SpeciesBovine
LotR38625
Other OAT NBP1-83239
Enlarge
5
reviewed by:
Verified Customer
Blocking test with OAT Recombinant Protein Antigen. Bovine 01/31/2019
View

Summary

ApplicationOther
Sample Testedcow liver tissue,cow liver tissues
SpeciesBovine
LotR38625

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for OAT Antibody (NBP1-83239) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional OAT Products

Bioinformatics Tool for OAT Antibody (NBP1-83239)

Discover related pathways, diseases and genes to OAT Antibody (NBP1-83239). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for OAT Antibody (NBP1-83239)

Discover more about diseases related to OAT Antibody (NBP1-83239).
 

Pathways for OAT Antibody (NBP1-83239)

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PTMs for OAT Antibody (NBP1-83239)

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Blogs on OAT

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
02/01/2019
Application: WB
Species: Bovine

Verified Customer
01/31/2019
Application: Blocking test with OAT Recombinant Protein Antigen.
Species: Bovine

Bioinformatics

Gene Symbol OAT