APRIL/TNFSF13 Antibody (Aprily-8)

Images

 
Western Blot: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL.Method: 10ug of ...read more
Immunohistochemistry-Paraffin: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Immunostaining of HEK 293 cells transfected with a human APRIL expression plasmid (left panels), or mock transfected (right panel) by ...read more
Flow Cytometry: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Analysis of cells with MAb to APRIL (Aprily-8).Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
Aprily-8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

APRIL/TNFSF13 Antibody (Aprily-8) Summary

Immunogen
Recombinant human APRIL (aa 93-233).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNFSF13
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This antibody is useful in Flow Cytometry, Immunocytochemistry (excellent), Immunohistochemistry (paraffin sections), Immunoprecipitation, Western Blot (excellent).
Publications
Read Publications using NBP1-97587.

Reactivity Notes

Recognizes Human APRIL and TWE-PRIL. Does not cross-react with Mouse APRIL and TWE-PRIL.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Notes

Method used for IHC-P: 3 days after transfection of cells with the indicated constructs, cells were fixed with 4% formaldehyde 5 min. at RT. After a wash in PBS, samples were dehydrated by washes in 60%, 80%, 90%, 100% EtOH and xylol. Cells were then dried and embedded in paraffin. Sections were cut, mounted on slides and dried overnight at 50C. Slides were then successively washed 2x 10 min. in xylol, 2x 10 min. in 100% ethanol, and then treated 10 min. in 100% methanol/0.6% H202 to inhibit endogenous peroxidase. Samples were rehydrated by washes in 90%, 80%, 60% ethanol and PBS. After micro-wave treatment, slides were washed 3x in PBS, blocked with IgG, and incubated for 1 hour with 5ug/ml Aprily-8 or control mouse IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After PBS washes, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.

Alternate Names for APRIL/TNFSF13 Antibody (Aprily-8)

  • A proliferation-inducing ligand
  • APRIL
  • APRILFLJ57090
  • CD256 antigen
  • CD256
  • TALL2
  • TALL-2
  • TNF- and APOL-related leukocyte expressed ligand 2
  • TNF-related death ligand 1
  • TNFSF13
  • TRDL1
  • TRDL-1
  • tumor necrosis factor (ligand) superfamily, member 13
  • tumor necrosis factor ligand superfamily member 13
  • tumor necrosis factor-like protein ZTNF2
  • tumor necrosis factor-related death ligand-1
  • ZTNF2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for APRIL/TNFSF13 Antibody (NBP1-97587)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for APRIL/TNFSF13 Antibody (NBP1-97587)

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Diseases for APRIL/TNFSF13 Antibody (NBP1-97587)

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PTMs for APRIL/TNFSF13 Antibody (NBP1-97587)

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Bioinformatics

Gene Symbol TNFSF13
Entrez