Home » BCMA/TNFRSF17 » BCMA/TNFRSF17 Antibodies » BCMA/TNFRSF17 Antibody (Vicky-1)

BCMA/TNFRSF17 Antibody (Vicky-1)

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 (1 Review)
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Immunohistochemistry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Immunostaining of HEK 293 cells transfected with a human BCMA expression plasmid (left panel), or mock transfected (right panel). Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone or 4% formaldehyde for 5 min. at room temperature. Slides were blocked with normal IgG, and incubated for 1 hour with 5ug/ml MAb to BCMA (human) (Vicky-1) in 1% BSA in 1x PBS. After washes in PBS, samples were incubated with the secondary antibody for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.
Immunohistochemistry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Immunostaining of HEK 293 cells transfected with a human BCMA expression plasmid (left panel), or mock transfected (right panel). Method: 3 days ...read more
Flow Cytometry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Analysis using the PE conjugate of NBP1-97637. Staining of 10^6 U266 cells using BCMA (human), mAb (Vicky-1) (PE conjugate) at a concentration of 10ug/ml.
Flow Cytometry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Detection of endogenous human BCMA with MAb to BCMA (human) (Vicky-1). Method: U266 cells (2x10^5) were incubated on ice for 30 min. with 0.2ug of MAb to ...read more
Flow Cytometry: BCMA/TNFRSF17 Antibody (Vicky-1) [NBP1-97637] - Analysis using the FITC conjugate of NBP1-97637. Staining of 10^6 Jurkat cells using BCMA (human), mAb (Vicky-1) (FITC conjugate) at a concentration of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
Vicky-1
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-97637AF350
Alexa Fluor 405
NBP1-97637AF405-0.1ml
Alexa Fluor 488
NBP1-97637AF488-0.1ml
Alexa Fluor 532
NBP1-97637AF532
Alexa Fluor 594
NBP1-97637AF594
Alexa Fluor 647
NBP1-97637AF647-0.1ml
Alexa Fluor 700
NBP1-97637AF700-0.1ml
Alexa Fluor 750
NBP1-97637AF750
Allophycocyanin
NBP1-97637APC-0.1ml
Allophycocyanin/Cy7
NBP1-97637APCCY7
Biotin
NBP1-97637B
DyLight 350
NBP1-97637UV-0.1ml
DyLight 405
NBP1-97637V-0.1ml
DyLight 488
NBP1-97637G-0.1ml
DyLight 550
NBP1-97637R
DyLight 594
NBP1-97637DL594
DyLight 650
NBP1-97637C
DyLight 680
NBP1-97637FR-0.1ml
DyLight 755
NBP1-97637IR-0.1ml
FITC
NBP1-97655-100Tests
HRP
NBP1-97637H
Janelia Fluor 549
NBP1-97637JF549
Janelia Fluor 646
NBP1-97637JF646
PE
NBP1-97656-0.1mg
PE
NBP1-97637PE-0.1ml
PE
NBP1-97656-0.1ml
PE/Atto594
NBP1-97637PEATT594
PE/Cy5.5
NBP1-97637PECY55
PE/Cy7
NBP1-97637PECY7
PerCP
NBP1-97637PCP-0.1ml
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BCMA/TNFRSF17 Antibody (Vicky-1) Summary

Immunogen
Recombinant human BCMA extracellular domain (aa 2-54).
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
TNFRSF17
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
Application Notes
Use in WB was reported in the scientific literature (PMID: 20454508). Functional Application: blocks binding of BAFF to human BCMA.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-97637 in the following applications:
Publications
Read Publications using NBP1-97637.

Reactivity Notes

Does not cross-react with Mouse BCMA.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for BCMA/TNFRSF17 Antibody (Vicky-1)

  • B cell maturation antigen
  • B-cell maturation protein
  • BCMA
  • BCMAtumor necrosis factor receptor superfamily member 17
  • BCMB-cell maturation factor
  • CD269 antigen
  • CD269
  • TNFRSF13A
  • TNFRSF17
  • tumor necrosis factor receptor superfamily, member 17

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: ABCA4 Antibody (3F4) [NBP1-30032] - Characterization of ABCA4 mRNA expression and western blot analyses of ABCA4 protein levels in the canine retina. Western blot analyses of ABCA4 (above), GAPDH (middle), and RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY licence.
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BCMA/TNFRSF17
Antibody (Vicky-1)
NBP1-97637
BCMA/TNFRSF17 Antibody (Vicky-1)
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC

Publications for BCMA/TNFRSF17 Antibody (NBP1-97637)(3)


Review for BCMA/TNFRSF17 Antibody (NBP1-97637) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-97637:
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Images Ratings Applications Species Date Details
Flow Cytometry BCMA/TNFRSF17 NBP1-97637
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reviewed by:
Anonymous
 Flow Human 01/27/2017
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Summary

ApplicationFlow Cytometry
Sample TestedRaji human Burkitt's lymphoma cell line,H929 cells
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BCMA/TNFRSF17 Antibody (NBP1-97637). (Showing 1 - 1 of 1 FAQs).

  1. What would be the appropriate negative isotype control for IHC on paraffin embedded human tissue?
    • A rat IgG1 isotype control is what you will want to use for NBP1-97637. Rat IgG1 contains a light chain and in rat, 99% of antibodies are kappa light chain, so this is what you will want to use. Here is a link to suitable isotype controls.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-97637AF350
Alexa Fluor 405 NBP1-97637AF405
Alexa Fluor 488 NBP1-97637AF488
Alexa Fluor 532 NBP1-97637AF532
Alexa Fluor 594 NBP1-97637AF594
Alexa Fluor 647 NBP1-97637AF647
Alexa Fluor 700 NBP1-97637AF700
Alexa Fluor 750 NBP1-97637AF750
Allophycocyanin NBP1-97637APC
Allophycocyanin/Cy7 NBP1-97637APCCY7
Biotin NBP1-97637B
DyLight 350 NBP1-97637UV
DyLight 405 NBP1-97637V
DyLight 488 NBP1-97637G
DyLight 550 NBP1-97637R
DyLight 594 NBP1-97637DL594
DyLight 650 NBP1-97637C
DyLight 680 NBP1-97637FR
DyLight 755 NBP1-97637IR
FITC NBP1-97655
HRP NBP1-97637H
Janelia Fluor 549 NBP1-97637JF549
Janelia Fluor 646 NBP1-97637JF646
PE NBP1-97637PE
PE NBP1-97656
PE NBP1-97656
PE/Atto594 NBP1-97637PEATT594
PE/Cy5.5 NBP1-97637PECY55
PE/Cy7 NBP1-97637PECY7
PerCP NBP1-97637PCP
Learn about Custom Antibody Labeling

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Recent Reviews

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Flow Cytometry BCMA/TNFRSF17 NBP1-97637
Enlarge
 Anonymous
01/27/2017
Application: Flow
Species: Human
read this review

Bioinformatics

Gene Symbol TNFRSF17
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