CAMLG Antibody (OTI1A3)

Western Blot: CAMLG Antibody (1A3) [NBP2-01801] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAMLG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAMLG.

Immunocytochemistry/Immunofluorescence: CAMLG Antibody (1A3) [NBP2-01801] Staining of COS7 cells transiently transfected by pCMV6-ENTRY CAMLG.

Immunohistochemistry-Paraffin: CAMLG Antibody (1A3) [NBP2-01801] - Staining of paraffin-embedded Human Kidney tissue using anti-CAMLG mouse monoclonal antibody.

Flow Cytometry: CAMLG Antibody (1A3) [NBP2-01801] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CAMLG antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: CAMLG Antibody (1A3) [NBP2-01801] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CAMLG mouse monoclonal antibody.

Immunohistochemistry-Paraffin: CAMLG Antibody (1A3) [NBP2-01801] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CAMLG mouse monoclonal antibody.

Immunohistochemistry-Paraffin: CAMLG Antibody (1A3) [NBP2-01801] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CAMLG mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.58 mg/ml

CAMLG Antibody (OTI1A3) Summary

• Description: Novus Biologicals Mouse CAMLG Antibody (OTI1A3) (NBP2-01801) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human CAMLG(NP_001736) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAMLG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 32.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.58 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CAMLG Antibody (OTI1A3)

• calcium modulating ligand
• calcium signal-modulating cyclophilin ligand
• calcium-signal modulating cyclophilin ligand
• CAMLcalcium-modulating cyclophilin ligand
• cyclophilin B-binding protein
• MGC163197

Background

The immunosuppressant drug cyclosporin A blocks a calcium-dependent signal from the T-cell receptor (TCR) that normally leads to T-cell activation. When bound to cyclophilin B, cyclosporin A binds and inactivates the key signaling intermediate calcineurin. The protein encoded by this gene functions similarly to cyclosporin A, binding to cyclophilin B and acting downstream of the TCR and upstream of calcineurin by causing an influx of calcium. This integral membrane protein appears to be a new participant in the calcium signal transduction pathway, implicating cyclophilin B in calcium signaling, even in the absence of cyclosporin. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAMLG
• Entrez:
  • 819(Human)