APRIL/TNFSF13 Antibodies

MAB8844) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> MAB8844) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Human APRIL/TNFSF13 protein was serially diluted 2-fold and captured by Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (MAB8844) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

AB-108-C, open histogram) followed by anti-Goat IgG PE-conjugated Secondary Antibody (F0107). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> AB-108-C, open histogram) followed by anti-Goat IgG PE-conjugated Secondary Antibody (F0107). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

AR002). Proliferation elicited by Recombinant Human APRIL/TNFSF13 (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB5860). The ND₅₀ is typically 0.15-0.75 µg/mL." class="big_lightbox"> AR002). Proliferation elicited by Recombinant Human APRIL/TNFSF13 (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB5860). The ND₅₀ is typically 0.15-0.75 µg/mL." alt="Recombinant Human APRIL/TNFSF13 induces proliferation in mouse B cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human APRIL/TNFSF13 (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB5860). The ND₅₀ is typically 0.15-0.75 µg/mL." class="big_thumb" />

204-IL) and 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for three days followed by a 3 hour incubation with monensin were stained with Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB8843) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # MAB004). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> 204-IL) and 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for three days followed by a 3 hour incubation with monensin were stained with Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB8843) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # MAB004). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="Human peripheral blood monocytes stimulated with 5 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for three days followed by a 3 hour incubation with monensin were stained with Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB8843) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # MAB004). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (AF884 ) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (AF884 ) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Human APRIL/TNFSF13 protein was serially diluted 2-fold and captured by Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB8844) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (AF884 ) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />