APAF-1 Antibody - BSA Free

Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-89050] - Analysis in human lymph node and pancreas tissues. Corresponding APAF1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-89050] - Staining of human pancreas shows weak to moderate positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-89050] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-89050] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in germinal center cells.

Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-89050] - Staining of human lymph node shows moderate to strong cytoplasmic positivity in germinal center cells.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

APAF-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit APAF-1 Antibody - BSA Free (NBP1-89050) is a polyclonal antibody validated for use in IHC. Anti-APAF-1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDILQEFVNKSLLFCDR
• Predicted Species: Mouse (95%), Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APAF1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
• Application Notes: IHC reported in scientific literature (PMID: 26259033). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  APAF-1 Protein (NBP1-89050PEP)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26259033).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for APAF-1 Antibody - BSA Free

• APAF1
• APAF-1
• APAF-1apoptotic protease activating factor 1
• apoptotic peptidase activating factor 1
• apoptotic peptidase activating factor
• apoptotic protease activating factor
• apoptotic protease-activating factor 1
• CED4
• DKFZp781B1145
• KIAA0413

Background

Apaf 1 (apoptosis protease-activating factor-1) is a key regulator of the mitochondrial apoptotic pathway (reviewed in Campioni et al, 2005), being the central element of the multimeric apoptosome. The apoptosome consists of cytochrome c, procaspase-9 and seven Apaf 1 monomers, and is considered to be core apoptotic machinery that executes mitochondria-dependent apoptosis. Cytochrome c, normally compartmentalized in the mitochorndria, is released into the cytoplasm following apoptotic stimuli. Apaf 1 binds cytochrome c in the cytoplasm and in the presence of dATP/ATP forms the apoptosome. The apoptosome binds procaspase-9 and promotes its autocatalytic activation. Active caspase-9, in turn, activates downstream caspases including 3, 6, and 7 contributing to the proteolytic caspase activation cascade which leads to cell death. Apaf 1 is an approx. 130 kDa protein. Alternative Apaf 1 splicing isoforms of Apaf 1 have been identified in mammalian cells, each having either or both an 11 amino acid insertion in the N-terminus and an additional WD40 repeat (43 aa) in the C-terminus; please refer to AceView of NCBI (http://www.ncbi.nih.gov/IEB/Research/Acembly/) for detailed information. Additionally, Apaf 1 can be proteolytically processed by active caspase-3 during apoptosis resulting in an Apaf 1 cleavage fragment of ~84 kDa (Lauber et al. 2001).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APAF-1 Antibody (NBP1-89050)(1)

Publication using NBP1-89050

• Nemanja Rodic, Jared P Steranka, Alvin Makohon-Moore et al. Retrotransposon insertions in the clonal evolution of pancreatic ductal adenocarcinoma. Nature Medicine 2015-08-10 [PMID: 26259033] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: APAF1
• Entrez:
  • 317(Human)
• Uniprot:
  • O14727(Human)