APAF-1 Products

Antibodies
APAF-1 Antibody (94408)
APAF-1 Antibody (94408)
MAB868
Species: Hu
Applications: WB
Host: Mouse Monoclonal
Formulation Catalog # Availability Price  
APAF-1 Antibody
APAF-1 Antibody
NB100-56074
Species: Hu, Mu, Rt, Ma
Applications: WB, ICC/IF, IHC, IP
Host: Rabbit Polyclonal
APAF-1 Antibody - BSA Free
APAF-1 Antibody - BSA Free
NBP1-77000
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IP
Host: Rabbit Polyclonal
Formulation Catalog # Availability Price  
ELISA Kits
Mouse APAF-1 ELISA Kit (Color ...
Mouse APAF-1 ELISA Kit (Colorimetric)
NBP2-68233
Species: Mu
Applications: ELISA
Human APAF-1 ELISA Kit (Color ...
Human APAF-1 ELISA Kit (Colorimetric)
NBP2-68232
Species: Hu
Applications: ELISA
Human APAF-1 ELISA Kit (Chemi ...
Human APAF-1 ELISA Kit (Chemilumin...
NBP2-68231
Species: Hu
Applications: ELISA
Lysates
APAF-1 Overexpression Lysate
APAF-1 Overexpression Lysate
NBP2-04870
Species: Hu
Applications: WB
Proteins
Recombinant Human APAF-1 GST ...
Recombinant Human APAF-1 GST (N-Te...
H00000317-Q01
Species: Hu
Applications: WB, ELISA, MA, AP
APAF-1 Antibody Blocking Pept ...
APAF-1 Antibody Blocking Peptide
NBP1-77000PEP
Species: Hu
Applications: AC
APAF-1 Recombinant Protein An ...
APAF-1 Recombinant Protein Antigen
NBP1-89050PEP
Species: Hu
Applications: AC

Description

Apaf 1 (apoptosis protease-activating factor-1) is a key regulator of the mitochondrial apoptotic pathway (reviewed in Campioni et al, 2005), being the central element of the multimeric apoptosome. The apoptosome consists of cytochrome c, procaspase-9 and seven Apaf 1 monomers, and is considered to be core apoptotic machinery that executes mitochondria-dependent apoptosis. Cytochrome c, normally compartmentalized in the mitochorndria, is released into the cytoplasm following apoptotic stimuli. Apaf 1 binds cytochrome c in the cytoplasm and in the presence of dATP/ATP forms the apoptosome. The apoptosome binds procaspase-9 and promotes its autocatalytic activation. Active caspase-9, in turn, activates downstream caspases including 3, 6, and 7 contributing to the proteolytic caspase activation cascade which leads to cell death. Apaf 1 is an approx. 130 kDa protein. Alternative Apaf 1 splicing isoforms of Apaf 1 have been identified in mammalian cells, each having either or both an 11 amino acid insertion in the N-terminus and an additional WD40 repeat (43 aa) in the C-terminus; please refer to AceView of NCBI (http://www.ncbi.nih.gov/IEB/Research/Acembly/) for detailed information. Additionally, Apaf 1 can be proteolytically processed by active caspase-3 during apoptosis resulting in an Apaf 1 cleavage fragment of ~84 kDa (Lauber et al. 2001).

Bioinformatics

Entrez Mouse
Human
Uniprot Human
Human
Human
Human
Human
Human
Human
Alternate Names
  • APAF-1apoptotic protease activating factor 1
  • apoptotic peptidase activating factor 1
  • apoptotic peptidase activating factor
  • apoptotic protease activating factor
  • apoptotic protease-activating factor 1
  • CED4
  • DKFZp781B1145
  • KIAA0413

Research Areas for APAF-1

Find related products by research area and learn more about each of the different research areas below.

Apoptosis
Cancer
Cell Biology
Mitochondrial Markers
Tumor Suppressors