FLASH Antibody

Images

 
Western Blot: FLASH Antibody [NBP1-77301] - Analysis of FLASH in HeLa whole cell lysate with NBP1-77301 at 0.5 ug/ml.
Immunocytochemistry/ Immunofluorescence: FLASH Antibody [NBP1-77301] - Immunocytochemistry of FLASH in HeLa cells using NBP1-77301 at 10 ug/ml.
Immunofluorescence: FLASH Antibody [NBP1-77301] - Immunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 ug/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

FLASH Antibody Summary

Immunogen
Antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human FLASH which differ from those of mouse by one amino acid. The immunogen is located within the last 50 amino acids of FLASH.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP8AP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunofluorescence 10 ug/ml
Application Notes
FLASH antibody can be used for detection of FLASH by Western blot at 0.25 to 0.5 mg/ml. An approximate 220 kDa band can be detected.
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
FLASH Peptide (NBP1-77301PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FLASH Antibody

  • CASP8 associated protein 2
  • caspase 8 associated protein 2
  • CED-4
  • FLASHKIAA1315CASP8-associated protein 2
  • FLICE associated huge
  • FLICE-associated huge protein
  • FLJ11208
  • human FLASH
  • RIP25FLASH homolog RIP25

Background

A novel mammalian CED-4 homologous was recently identified and cloned in mouse and human and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity. FLASH is widely expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional FLASH Products

FLASH NBP1-77301

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Bioinformatics

Gene Symbol CASP8AP2
Uniprot