FLASH Antibody


Western Blot: FLASH Antibody [NBP1-77301] - Analysis of FLASH in HeLa whole cell lysate with NBP1-77301 at 0.5 ug/ml.
Immunocytochemistry/ Immunofluorescence: FLASH Antibody [NBP1-77301] - Immunocytochemistry of FLASH in HeLa cells using NBP1-77301 at 10 ug/ml.
Immunofluorescence: FLASH Antibody [NBP1-77301] - Immunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 ug/mL.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IF
1 mg/ml

Order Details

FLASH Antibody Summary

Antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy-terminus of human FLASH which differ from those of mouse by one amino acid.
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunofluorescence 10 ug/ml
Application Notes
FLASH antibody can be used for detection of FLASH by Western blot at 0.25 to 0.5 mg/ml. An approximate 220 kDa band can be detected.
Control Peptide
FLASH Peptide (NBP1-77301PEP)

Reactivity Notes


Alternate Names for FLASH Antibody

  • CASP8 associated protein 2
  • caspase 8 associated protein 2
  • CED-4
  • FLASHKIAA1315CASP8-associated protein 2
  • FLICE associated huge
  • FLICE-associated huge protein
  • FLJ11208
  • human FLASH
  • RIP25FLASH homolog RIP25


A novel mammalian CED-4 homologous was recently identified and cloned in mouse and human and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity. FLASH is widely expressed.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FLASH Antibody (NBP1-77301) (0)

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Secondary Antibodies


Isotype Controls

Additional FLASH Antibody Products

FLASH NBP1-77301

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Blogs on FLASH

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Contact Information

Product PDFs


Gene Symbol CASP8AP2

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