FLASH Antibody - BSA Free

Western Blot: FLASH Antibody [NBP1-77301] - Analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 ug/ml.

Immunocytochemistry/ Immunofluorescence: FLASH Antibody - BSA Free [NBP1-77301] - Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 u/mL.

Immunocytochemistry/ Immunofluorescence: FLASH Antibody - BSA Free [NBP1-77301] - Immunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

FLASH Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human FLASH which differ from those of mouse by one amino acid. The immunogen is located within the last 50 amino acids of FLASH.
• Predicted Species: Mouse (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP8AP2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Western Blot 0.5 ug/ml
• Application Notes: An approximate 220 kDa band can be detected in Western Blot.
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  FLASH Peptide (NBP1-77301PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for FLASH Antibody - BSA Free

• CASP8 associated protein 2
• caspase 8 associated protein 2
• CED-4
• FLASHKIAA1315CASP8-associated protein 2
• FLICE associated huge
• FLICE-associated huge protein
• FLJ11208
• human FLASH
• RIP25FLASH homolog RIP25

Background

A novel mammalian CED-4 homologous was recently identified and cloned in mouse and human and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity. FLASH is widely expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASP8AP2
• Uniprot:
  • AF165161(Human)