Angiopoietin-like Protein 3/ANGPTL3 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Angiopoietin-like Protein 3/ANGPTL3 Antibody [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Angiopoietin-like 3 (R&D Systems, Catalog # 3829-AN)
  Ser17-Glu460
  Accession # Q9Y5C1
• Specificity: Detects human Angiopoietin-like 3 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross‑reactivity with recombinant human (rh) Angiopoietin-1, rhAngiopoietin-2, rhAngiopoietin-4, rhANGPTL4, recombinant mouse (rm) Angiopoietin-3, rmANGPTL3 and rmANGPTL4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ANGPTL3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human Angiopoietin-like 3 Antibody (Catalog # MAB38291)
  ELISA Detection: Human Angiopoietin-like Protein 3/ANGPTL3 Biotinylated Antibody (Catalog # BAF3829)
  Standard: Recombinant Human Angiopoietin-like Protein 3/ANGPTL3 (Catalog # 3829-AN)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-like Protein 3/ANGPTL3 Antibody [Biotin]

• AGNPT5
• Ang-5
• angiopoietin 5
• Angiopoietin-5
• angiopoietin-like 3
• Angiopoietin-like Protein 3
• angiopoietin-related protein 3
• ANGPT5angiopoietin-5
• ANGPTL3
• FHBL2

Background

Angiopoietin-like 3 (ANGPTL3), also known as angiopoietin-5, is a secreted, hepatic angiopoietin-like glycoprotein that is structurally related to angiopoietins. Human ANGPTL3 contains an N‑terminal heparin-binding motif, a coiled-coil domain (CCD) that mediates the formation of non-covalent higher-order oligomers, and a C‑terminal fibrinogen-like domain that binds integrin  alpha _v beta ₃. In vivo, ANGPTL3 is proteolytically cleaved between the CCD and the fibrinogen-like domain, generating an N‑terminal fragment that retains the inhibitory activity on lipoprotein lipase and a C‑terminal fragment that can induce angiogenesis. Full length and cleaved ANGPTL3 circulate in plasma. Mature human ANGPTL3 shares 76% and 83% aa sequence identity with mouse and porcine ANGPTL3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-like Protein 3/ANGPTL3 Antibody (BAF3829)(2)

Publications using BAF3829

• Kraaijenhof, JM;Tromp, TR;Nurmohamed, NS;Reeskamp, LF;Langenkamp, M;Levels, JHM;Boekholdt, SM;Wareham, NJ;Hoekstra, M;Stroes, ESG;Hovingh, GK;Grefhorst, A; ANGPTL3 (Angiopoietin-Like 3) Preferentially Resides on High-Density Lipoprotein in the Human Circulation, Affecting Its Activity Journal of the American Heart Association 2023-10-27 [PMID: 37889183] (Western Blot, Human)
• Probing isoform-specific functions of polypeptide GalNAc-transferases using zinc finger nuclease glycoengineered SimpleCells. Proc Natl Acad Sci U S A, 2012-05-07;109(25):9893-8. 2012-05-07 [PMID: 22566642] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ANGPTL3
• Uniprot:
  • Q9Y5C1(Human)