Angiopoietin-2 Antibody (748246) [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: WB
• Clone: 748246
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

Angiopoietin-2 Antibody (748246) [Alexa Fluor® 488] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Angiopoietin-2
  Asp68-Phe496
  Accession # O35608
• Specificity: Detects mouse Angiopoietin-2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Angiopoietin-1, rmAngiopoietin-3, recombinant human (rh) Angiopoietin-2, and rhAngiopoietin-4
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-2 Antibody (748246) [Alexa Fluor® 488]

• AGPT2
• ANG2
• ANG-2
• angiopoietin 2
• Angiopoietin-2
• angiopoietin-2a
• angiopoietin-2B
• angiopoitin 2
• ANGPT2
• Tie2-ligand

Background

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted 66-70 kDa glycoprotein member of the angiopoietin family of growth factors. It is expressed by endothelial cells, macrophages and skeletal muscle fibers, and binds to the Tie2 receptor on select cell types, including endothelial cells and monocytes. Ang-2 has multiple functions. In the presence of growth factors, it promotes angiogenesis; in the absence of growth factors, it induces vascular regression. It is generally considered to be an antagonist of Ang-1 activity. Mature mouse Ang-2 is 478 amino acids (aa) in length (aa 19-496). It contains one coiled-coil region (aa 159-256) plus a C-terminal Fibrinogen-like domain (aa 275-495). Mouse Ang-2 undergoes covalent oligomerization, forming 140 kDa homodimers and 280 kDa homotetramers. The higher-order oligomers are strong Tie-2 agonists. Over aa 68-496, mouse Ang-2 shares 87% and 96% aa sequence identity with human and rat Ang-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O35608(Mouse)