Angiopoietin-1 Antibody (MM0019-26B10)

Immunohistochemistry-Paraffin: Angiopoietin-1 Antibody (MM0019-26B10) [NB110-85464] - Angiopoietin 1 Antibody (MM0019-26B10) [NB110-85464] - Formalin fixed and paraffin embedded human placental tissue section is subjected to IHC with anti human Angiopoietin-1.

• Reactivity: Hu, Ch
• Applications: WB, IHC, IHC-Fr, IHC-P
• Clone: MM0019-26B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Angiopoietin-1 Antibody (MM0019-26B10) Summary

• Description: PLEASE NOTE: 0.02mg sample size is provided in reconstituted format and the 0.1mg size is provided lyophilized. (Please see reconstitution instuctions).
• Immunogen: Purified human recombinant human Angiopoietin-1.
• Specificity: This recognizes human Ang1 without cross reactivity with human Ang2.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANGPT1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50
  • Immunohistochemistry-Paraffin 1:50-1:400
• Application Notes: This antibody recognizes human Ang1 in western blot and IHC-Paraffin. For use in FFPE tissue, our recommended antigen retrival-treat the paraffin section with 20ug/ml Proteinase K (in PBS) for 15-25min.
• Control:

  Angiopoietin-1 Overexpression Lysate

Reactivity Notes

Chicken data from customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: A 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with 0.5 ml sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for Angiopoietin-1 Antibody (MM0019-26B10)

• AGP1
• AGPT
• Ang1
• ANG-1
• angiopoietin 1
• Angiopoietin-1
• ANGPT1
• KIAA0003

Background

Angiopoietin 1 is one of the Angiopoietin (Ang) family of growth factors. It binds and activates the TIE2 receptor tyrosine kinase by inducing its tyrosine phosphorylation. It is implicated in endothelial developmental processes later and distinct from that of VEGF. The structure of angiopoietin-1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. Angiopoietin 1 appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. It also mediates blood vessel maturation/stability and it may play an important role in the heart early development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-1 Antibody (NB110-85464)(1)

Publication using NB110-85464

• Regula JT, Lundh von Leithner P, Foxton R et al. Targeting key angiogenic pathways with a bispecific CrossMAb optimized for neovascular eye diseases. EMBO Mol Med Nov 2 2016 [PMID: 27742718]

Reviews for Angiopoietin-1 Antibody (NB110-85464) (2)

Average Rating: 3

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Other.

reviewed by: Anony- mous IF Other 08/26/2014

Summary

• Application: Immunofluorescence
• Species: Other
• File: View PDF

reviewed by: Corey. IHC-Fr Human 07/12/2013

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Frozen human skin sections
• Species: Human

Blocking

• Blocking Details: IgG-free BSA

Primary Anitbody

• Dilution Ratio: 1:50 in PBS/1% IgG-free BSA, over night at 4C

Secondary Antibody

• Secondary Description: donkey anti-mouse IgG, conjugated to Rhodamine
• Secondary Manufacturer Cat#: Jackson 715-296-150
• Secondary Concentration: 1:250

Details

• Detection Notes: Nikon Eclipse E800. Original magnification 200x
• Wash Description: Washed for 30 min in PBS

FAQs for Angiopoietin-1 Antibody (NB110-85464). (Showing 1 - 1 of 1 FAQ).

1. How do you dissolve the lyophilized Ab? Water or PBS? The datasheet for NB110-85464 just says "Buffer: Lyophilized" - - in Water? Buffer? Concentration of Buffer? NB110-85467 says Lyophilized from a solution of PBS - again how to dissolve the Ab - which PBS and what concentration?
   • The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). 100 ug antibody is lyophilized from 40 ul of 1 x PBS. We suggest reconstituting the antibody with 500 ul sterile 1 x PBS (or to be perfect, with 40 ul H2O plus 460 ul PBS), to give a final concentration of 200 ug/ml. Alternatively, you may reconstitute the antibody in 40 ul H2O if a small volume is preferred.

Bioinformatics

• Gene Symbol: ANGPT1
• Entrez:
  • 284(Human)
• Uniprot:
  • Q15389()