Angiopoietin-1 Antibody (MM0019-26B10)

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Immunohistochemistry-Paraffin: Angiopoietin-1 Antibody (MM0019-26B10) [NB110-85464] - Angiopoietin 1 Antibody (MM0019-26B10) [NB110-85464] - Formalin fixed and paraffin embedded human placental tissue section is ...read more

Product Details

Summary
Reactivity Hu, ChSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clone
MM0019-26B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Angiopoietin-1 Antibody (MM0019-26B10) Summary

Description
PLEASE NOTE: 0.02mg sample size is provided in reconstituted format and the 0.1mg size is provided lyophilized. (Please see reconstitution instuctions).
Immunogen
Purified human recombinant human Angiopoietin-1.
Specificity
This recognizes human Ang1 without cross reactivity with human Ang2.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ANGPT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50
  • Immunohistochemistry-Paraffin 1:50-1:400
Application Notes
This antibody recognizes human Ang1 in western blot and IHC-Paraffin. For use in FFPE tissue, our recommended antigen retrival-treat the paraffin section with 20ug/ml Proteinase K (in PBS) for 15-25min.
Positive Control
Angiopoietin-1 Lysate (NBL1-07521)
Reviewed Applications
Read 2 Reviews rated 3
using
NB110-85464 in the following applications:

Publications
Read Publication using NB110-85464.

Reactivity Notes

Chicken data from customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with 0.5 ml sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for Angiopoietin-1 Antibody (MM0019-26B10)

  • AGP1
  • AGPT
  • Ang1
  • ANG-1
  • angiopoietin 1
  • Angiopoietin-1
  • ANGPT1
  • KIAA0003

Background

Angiopoietin 1 is one of the Angiopoietin (Ang) family of growth factors. It binds and activates the TIE2 receptor tyrosine kinase by inducing its tyrosine phosphorylation. It is implicated in endothelial developmental processes later and distinct from that of VEGF. The structure of angiopoietin-1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. Angiopoietin 1 appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. It also mediates blood vessel maturation/stability and it may play an important role in the heart early development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Angiopoietin-1 Antibody (NB110-85464)(1)

Reviews for Angiopoietin-1 Antibody (NB110-85464) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.
We have 2 reviews tested in 2 application: IF, IHC-Fr.

Reviews using NB110-85464:
Filter by Applications
IF
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Angiopoietin-1 NB110-85464
Enlarge
reviewed by:
Anony- mous
IF Other 08/26/2014
View

Summary

ApplicationImmunofluorescence
SpeciesOther
FileView PDF
Immunohistochemistry-Frozen Angiopoietin-1 NB110-85464
Enlarge
reviewed by:
Corey.
IHC-Fr Human 07/12/2013
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen human skin sections
SpeciesHuman

Blocking

Blocking DetailsIgG-free BSA

Primary Anitbody

Dilution Ratio1:50 in PBS/1% IgG-free BSA, over night at 4C

Secondary Antibody

Secondary Descriptiondonkey anti-mouse IgG, conjugated to Rhodamine
Secondary Manufacturer Cat#Jackson 715-296-150
Secondary Concentration1:250

Details

Detection NotesNikon Eclipse E800. Original magnification 200x
Wash DescriptionWashed for 30 min in PBS

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Angiopoietin-1 Antibody (NB110-85464). (Showing 1 - 1 of 1 FAQ).

  1. How do you dissolve the lyophilized Ab? Water or PBS? The datasheet for NB110-85464 just says "Buffer: Lyophilized" - - in Water? Buffer? Concentration of Buffer? NB110-85467 says Lyophilized from a solution of PBS - again how to dissolve the Ab - which PBS and what concentration?
    • The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). 100 ug antibody is lyophilized from 40 ul of 1 x PBS. We suggest reconstituting the antibody with 500 ul sterile 1 x PBS (or to be perfect, with 40 ul H2O plus 460 ul PBS), to give a final concentration of 200 ug/ml. Alternatively, you may reconstitute the antibody in 40 ul H2O if a small volume is preferred.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Angiopoietin-1 Antibody (NB110-85464)

Discover related pathways, diseases and genes to Angiopoietin-1 Antibody (NB110-85464). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Angiopoietin-1

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Contact Information

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Calculators

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Recent Reviews

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5
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4
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2
2
0
1
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Anony- mous
08/26/2014
Application: IF
Species: Other

Corey.
07/12/2013
Application: IHC-Fr
Species: Human

Bioinformatics

Gene Symbol ANGPT1
Entrez
Uniprot