Angiopoietin-1 Antibody (892732)

Western blot shows lysates of mouse heart tissue and mouse kidney tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB8220) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin‑1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 892732
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Angiopoietin-1 Antibody (892732) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Angiopoietin‑1
  Ser20-Phe498
  Accession # O08538
• Specificity: Detects mouse Angiopoietin‑1 in ELISA and Western Blot. In ELISA, no cross-reactivity with recombinant mouse Angiopoietin‑2 is detected.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: ANGPT1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-1 Antibody (892732)

• AGP1
• AGPT
• ANG1
• ANG-1
• angiopoietin 1
• Angiopoietin-1
• ANGPT1
• KIAA0003

Background

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted ligands which bind with similar affinity to Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature (1).

Ang-1 cDNA encodes a 498 amino acid (aa) residue precursor protein that contains a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-1 shares approximately 97% and 60% amino acid sequence identity with mouse Ang-1 and human Ang-2, respectively (1, 2). Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context (5).

1. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
2. Davis, S. et al. (1996) Cell 87:1161.
3. Witzenbichler, B. et al. (1998) J. Biol. Chem. 273:18514.
4. Papapetropoulos, A. et al. (1999) Lab. Inest. 79:213.
5. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-1 Antibody (MAB8220)(1)

Publication using MAB8220

• C Ma, Q Wei, B Cao, X Cheng, J Tian, H Pu, A Yusufu, L Cao A multifunctional bioactive material that stimulates osteogenesis and promotes the vascularization bone marrow stem cells and their resistance to bacterial infection PLoS ONE, 2017;12(3):e0172499. 2017 [PMID: 28358890] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: ANGPT1
• Uniprot:
  • O08538()