Angiopoietin-1 Antibody (892732) [Alexa Fluor® 405]

• Reactivity: Mu
• Applications: WB
• Clone: 892732
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 405

Angiopoietin-1 Antibody (892732) [Alexa Fluor® 405] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Angiopoietin‑1
  Ser20-Phe498
  Accession # O08538
• Specificity: Detects mouse Angiopoietin‑1 in ELISA and Western Blot. In ELISA, no cross-reactivity with recombinant mouse Angiopoietin‑2 is detected.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-1 Antibody (892732) [Alexa Fluor® 405]

• AGP1
• AGPT
• Ang1
• ANG-1
• angiopoietin 1
• Angiopoietin-1
• ANGPT1
• KIAA0003

Background

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted ligands which bind with similar affinity to Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature (1).

Ang-1 cDNA encodes a 498 amino acid (aa) residue precursor protein that contains a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-1 shares approximately 97% and 60% amino acid sequence identity with mouse Ang-1 and human Ang-2, respectively (1, 2). Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.