![Western Blot: Angiogenin Antibody (HL2214) - Azide and BSA Free [NBP3-25360] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Angiogenin antibody [HL2214] (NBP3-25360) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25360_rabbit-angiogenin-mab-hl2214-azide-and-bsa-free-212024939157.jpg "Western Blot: Angiogenin Antibody (HL2214) - Azide and BSA Free [NBP3-25360] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Angiogenin antibody [HL2214] (NBP3-25360) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL2214 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Rabbit Angiogenin Antibody (HL2214) - Azide and BSA Free (NBP3-25360) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human Angiogenin. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ANG |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Angiogenin Antibody (NBP3-25360)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ANG |
![Immunohistochemistry-Paraffin: Angiogenin Antibody (HL2214) - Azide and BSA Free [NBP3-25360] - Angiogenin antibody [HL2214] detects Angiogenin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Angiogenin stained by Angiogenin antibody [HL2214] (NBP3-25360) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25360_rabbit-angiogenin-mab-hl2214-azide-and-bsa-free-2120249362764.jpg "Immunohistochemistry-Paraffin: Angiogenin Antibody (HL2214) - Azide and BSA Free [NBP3-25360] - Angiogenin antibody [HL2214] detects Angiogenin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Angiogenin stained by Angiogenin antibody [HL2214] (NBP3-25360) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: Angiogenin Antibody (HL2214) - Azide and BSA Free [NBP3-25360] - Angiogenin antibody [HL2214] detects Angiogenin protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Angiogenin stained by Angiogenin antibody [HL2214] (NBP3-25360) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25360_rabbit-angiogenin-mab-hl2214-azide-and-bsa-free-2120249351353.jpg "Immunocytochemistry/Immunofluorescence: Angiogenin Antibody (HL2214) - Azide and BSA Free [NBP3-25360] - Angiogenin antibody [HL2214] detects Angiogenin protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Angiogenin stained by Angiogenin antibody [HL2214] (NBP3-25360) diluted at 1:500. Blue: Fluoroshield with DAPI .")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")