Androgen R/NR3C4 [p Ser94] Antibody - Azide Free

Western Blot: Androgen R/NR3C4 [p Ser94] Antibody [NBP2-29527] - HeLa lysate showing specific immunolabeling of the ~110 kD AR protein phosphorylated at Ser94. The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen.

• Reactivity: Hu, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Androgen R/NR3C4 [p Ser94] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser94 conjugated to KLH. Accession # P10275
• Modification: p Ser94
• Specificity: Specific for endogenous levels of the ~110k Androgen R/NR3C4 protein phosphorylated at Ser94. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to generate the antibody but not by the corresponding dephospho-peptide.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AR
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Androgen R/NR3C4 [p Ser94] Antibody - Azide Free

• AIS
• Androgen R
• androgen receptor
• AndrogenR
• AR
• DHTR
• DHTRTFM
• Dihydrotestosterone receptorHYSP1
• HUMARA
• NR3C4
• NR3C4KD
• Nuclear receptor subfamily 3 group C member 4
• SBMA
• SMAX1
• SMAX1SBMA
• spinal and bulbar muscular atrophy

Background

The androgen receptor (AR) is a DNA-binding transcription factor that regulates genes critical for the development and maintenance of the male sexual phenotype. Defects in androgen receptor have been shown to play a role in prostate cancer, and inhibition of AR activity through modulation of signal transduction pathways may delay prostate cancer progression (Heinlein and Chang 2004). Multiple phosphorylation sites have been identified on the androgen receptor that affect cross-talk between growth factor signaling and androgen in prostate development and cancer (Gioeli et al., 2002). One of these sites, at Ser94, appears constitutively phosphorylated and exhibits no response to treatments with stimulating hormone (Gioeli et al., 2002). The site at Ser94 is unique among the AR phosphorylation sites in that it does not achieve a maximal level of phosphorylation between translation and the initial round of nuclear import, having a strong bias for androgen-independent phosphorylation in the cytoplasm (Kesler et al., 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AR
• Entrez:
  • 367(Human)
• Uniprot:
  • P10275()