Androgen R/NR3C4 Antibody

Images

 
Western Blot: Androgen R/NR3C4 Antibody [NBP2-15384] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Androgen Receptor antibody [N1], N-term diluted at 1:500. The ...read more
Immunocytochemistry/ Immunofluorescence: Androgen R/NR3C4 Antibody [NBP2-15384] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Androgen Receptor protein stained by Androgen Receptor antibody ...read more
Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody [NBP2-15384] - Mouse prostate. Androgen Receptor antibody [N1], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Rb, RM, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Androgen R/NR3C4 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Androgen Receptor. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%), Rabbit (100%), Rhesus Macaque (100%), Chicken (93%), Canine (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Androgen R/NR3C4 Antibody

  • AIS
  • Androgen R
  • androgen receptor
  • AndrogenR
  • AR
  • DHTR
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • NR3C4
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SBMA
  • SMAX1
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol AR
Uniprot