Androgen R/NR3C4 Antibody

Images

 
Western Blot: Androgen R/NR3C4 Antibody [NB100-1446] - (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody [NB100-1446] - (2ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Chromatin Immunoprecipitation: Androgen R/NR3C4 Antibody [NB100-1446] - (Protein G) measuring FKBP5 enrichment.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Androgen R/NR3C4 Antibody Summary

Immunogen
Peptide with sequence EVQLGLGRVYPRPPSC corresponding to N-Terminus according to NP_000035.2.
Localization
Nuclear
Specificity
This antibody is expected to recognize isoform 1 (NP_000035.2) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3-2 ug/ml
  • Chromatin Immunoprecipitation
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:64000
Application Notes
ChIP: A 6-fold increased presence of the FKBP5 locus observed when comparing the precipitations using Goat Anti-Androgen Receptor with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.
WB: Approx. 95 kDa band observed in human brain and human heart lysates (calculated MW of 99 kDa band according to NP_000035).
IHC-P: Human prostate shows nuclear staining in the secretory cells of the gland.
Theoretical MW
99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Heart Lysate (NB820-59217)
Brain Lysate (NB820-59177)
Publications
Read Publication using NB100-1446.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Androgen R/NR3C4 Antibody

  • AIS
  • Androgen R
  • androgen receptor
  • AndrogenR
  • AR
  • DHTR
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • NR3C4
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SBMA
  • SMAX1
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

Like all steroid receptors, the androgen receptor has several functional domains. Androgen receptors have a DNA binding domain, a hinge section, and the hormone binding domain. There is only one active form of the androgen receptor. Smaller forms represent proteolytic fragments that are not considered physiologically relevant. The androgen receptor is most closely related to the progesterone receptor, and progestins in higher dosages can block the androgen receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Androgen R/NR3C4 Antibody (NB100-1446)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for Androgen R/NR3C4 Antibody (NB100-1446) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AR
Entrez
Uniprot