Androgen R/NR3C4 Antibody

Western Blot: Androgen R/NR3C4 Antibody [NB100-1446] - Staining of Human Cerebellum (A) and (0.1ug/ml) negative control Pancreas (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Androgen R/NR3C4 Antibody [NB100-1446] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: Androgen R/NR3C4 Antibody [NB100-1446] - (2ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Chromatin Immunoprecipitation: Androgen R/NR3C4 Antibody [NB100-1446] - (Protein G) measuring FKBP5 enrichment.

Immunocytochemistry/Immunofluorescence: Androgen R/NR3C4 Antibody [NB100-1446] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] - Androgen receptor immunoreactivity in equine epididymis. NB100-1446 was diluted to 1 ug/mL and left on tissue sections for 30 min at room temperature. Immunoreactivity was best demonstrated with heat induced epitope retrieval using an EDTA based solution. Image from a verified customer review.

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] - Androgen receptor immunoreactivity in canine epididymis. NB100-1446 was diluted to 1 ug/mL and left on tissue sections for 30m at room temperature. Immunoreactivity was best demonstrated with heat induced epitope retrieval in an EDTA based buffer. Image from a verified customer review.

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] - Androgen receptor immunoreactivity in feline epididymis. NB100-1446 was diluted to 1 ug/mL and left on tissue sections for 30m at room temperature. Immunoreactivity was best demonstrated with heat induced epitope retrieval in an EDTA based buffer. Image from a verified customer review.

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] - Androgen receptor immunoreactivity in mouse testis. NB100-1446 was diluted to 1 ug/mL and left on tissue sections for 30m at room temperature. Immunoreactivity was best demonstrated with heat induced epitope retrieval in an EDTA based buffer. Image from a verified customer review.

Immunohistochemistry-Paraffin: Goat Polyclonal Androgen R/NR3C4 Antibody [IMGENEX: IMG-3238] [NB100-1446] - Androgen receptor immunoreactivity in human testis. NB100-1446 was diluted to 1ug per mL and left on tissue sections for 30m at room temperature. Immunoreactivity was best demonstrated with heat-induced epitope retrieval in an EDTA-based solution. Image from a verified customer review.

• Reactivity: Hu, Eq, Fe, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Androgen R/NR3C4 Antibody Summary

• Immunogen: Peptide with sequence EVQLGLGRVYPRPPSC corresponding to N-Terminus according to NP_000035.2.
• Localization: Nuclear
• Specificity: This antibody is expected to recognize isoform 1 (NP_000035.2) only.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2-3 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1-0.5ug/ml
• Application Notes: ChIP: A 6-fold increased presence of the FKBP5 locus observed when comparing the precipitations using Goat Anti-Androgen Receptor with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.
  WB: Approx. 95 kDa band observed in human brain and human heart lysates (calculated MW of 99 kDa band according to NP_000035).
  IHC-P: Human prostate shows nuclear staining in the secretory cells of the gland.
• Theoretical MW: 99 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Heart Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Feline and Equine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Androgen R/NR3C4 Antibody

• AIS
• Androgen R
• androgen receptor
• AndrogenR
• AR
• DHTR
• DHTRTFM
• Dihydrotestosterone receptorHYSP1
• HUMARA
• NR3C4
• NR3C4KD
• Nuclear receptor subfamily 3 group C member 4
• SBMA
• SMAX1
• SMAX1SBMA
• spinal and bulbar muscular atrophy

Background

Like all steroid receptors, the androgen receptor has several functional domains. Androgen receptors have a DNA binding domain, a hinge section, and the hormone binding domain. There is only one active form of the androgen receptor. Smaller forms represent proteolytic fragments that are not considered physiologically relevant. The androgen receptor is most closely related to the progesterone receptor, and progestins in higher dosages can block the androgen receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Androgen R/NR3C4 Antibody (NB100-1446)(3)

Publications using NB100-1446

• Zhang D, Fan J, Liu H et al. Testosterone enhances taurine synthesis by upregulating androgen receptor and cysteine sulfinic acid decarboxylase expressions in male mouse liver American journal of physiology. Gastrointestinal and liver physiology 2023-04-01 [PMID: 36749568]
• Zhang HM, Johansson LS. STM study of PTCDA on Sn/Si(111)-2√3×2√3. The Journal of chemical physics 2016-08-23 [PMID: 27036469]
• Trapman J, Klaassen P, Kuiper GG et al. Cloning, structure and expression of a cDNA encoding the human androgen receptor. Biochem Biophys Res Commun 1988-05-31 [PMID: 3377788]

Reviews for Androgen R/NR3C4 Antibody (NB100-1446) (5)

Average Rating: 4.8

(Based on 5 reviews)

We have 5 reviews tested in 5 species: Human, Mouse, Canine, Equine, Feline.

reviewed by: Verified Customer IHC-P Human 11/17/2023

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Testis
• Species: Human
• Lot: P1

reviewed by: Verified Customer IHC-P Equine 10/27/2023

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Epididymis
• Species: Equine
• Lot: P1

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

reviewed by: Verified Customer IHC-P Canine 10/27/2023

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Epididymis
• Species: Canine
• Lot: P1

reviewed by: Verified Customer IHC-P Feline 10/27/2023

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Epididymis
• Species: Feline
• Lot: P1

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

reviewed by: Verified Customer IHC-P Mouse 10/27/2023

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Testis
• Species: Mouse
• Lot: P1

Bioinformatics

• Gene Symbol: AR
• Entrez:
  • 367(Human)
  • 11835(Mouse)
  • 24208(Rat)
• Uniprot:
  • P10275()