AMPK alpha 2 Antibody

Images

 
Western Blot: AMPK alpha 2 Antibody [NBP1-49859] - staining of Mouse (A) and Rat (B) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: AMPK alpha 2 Antibody [NBP1-49859] (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA: AMPK alpha 2 Antibody [NBP1-49859] - (1.5ug/ml) as the reporter used with the capture rabbit antibody (2.5ug/ml).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, EIA, ELISA, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AMPK alpha 2 Antibody Summary

Immunogen
Peptide with sequence CPLDALNTTKP, from the internal region of the protein sequence according to NP_006243.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PRKAA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • ELISA 1:100-1:2000
  • Enzyme Immunoassay 1.5-3 ug/ml
  • Peptide ELISA 1:16000
Application Notes
Western blot: Approx 65kDa band observed in Human Heart and Liver lysates (calculated MW of 62.3kDa according to NP_006243.2). Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PRKAA2 captured by a rabbit antibody. Recommended reporter concentration: 1.5-3ug/ml
Publications
Read Publication using NBP1-49859.

Reactivity Notes

Cross reacts with Human, mouse and Rat. Expected from sequence similarity: Mouse, Rat, Dog, Pig and Cow.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AMPK alpha 2 Antibody

  • 5'-AMP-activated protein kinase catalytic subunit alpha-2
  • AMPK alpha 2
  • AMPK subunit alpha-2
  • AMPK2
  • AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain
  • AMPK-alpha-2 chain
  • EC 2.7.11
  • EC 2.7.11.1
  • PRKAA
  • PRKAA2
  • protein kinase, AMP-activated, alpha 2 catalytic subunit

Background

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AMPK alpha 2 Antibody (NBP1-49859)(1)

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Blogs on AMPK alpha 2.

AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

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Bioinformatics

Gene Symbol PRKAA2
Entrez
Uniprot