AMPK alpha 2 Antibody (1G8)

Images

 
Western Blot: AMPK alpha 2 Antibody (1G8) [H00005563-M02] - Analysis of PRKAA2 expression in transfected 293T cell line by PRKAA2 monoclonal antibody (M02), clone 1G8.Lane 1: PRKAA2 transfected lysate(62.3 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: AMPK alpha 2 Antibody (1G8) [H00005563-M02] - Analysis of monoclonal antibody to PRKAA2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: AMPK alpha 2 Antibody (1G8) [H00005563-M02] - Analysis of PRKAA2 over-expressed 293 cell line, cotransfected with PRKAA2 Validated Chimera RNAi ( Cat # H00005563-R01V ) (Lane 2) or non-transfected control ...read more
Proximity Ligation Assay: AMPK alpha 2 Antibody (1G8) [H00005563-M02] - Analysis of protein-protein interactions between STK11 and PRKAA2. HeLa cells were stained with anti-STK11 rabbit purified polyclonal 1:1200 and ...read more
ELISA: AMPK alpha 2 Antibody (1G8) [H00005563-M02] - Detection limit for recombinant GST tagged PRKAA2 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, PLA, RNAi
Clone
1G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

AMPK alpha 2 Antibody (1G8) Summary

Immunogen
PRKAA2 (NP_006243 453 a.a. - 552 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR
Specificity
PRKAA2 - protein kinase, AMP-activated, alpha 2 catalytic subunit
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PRKAA2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Proximity Ligation Assay
  • RNA Inhibition
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AMPK alpha 2 Antibody (1G8)

  • 5'-AMP-activated protein kinase catalytic subunit alpha-2
  • AMPK alpha 2
  • AMPK subunit alpha-2
  • AMPK2
  • AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain
  • AMPK-alpha-2 chain
  • EC 2.7.11
  • EC 2.7.11.1
  • PRKAA
  • PRKAA2
  • protein kinase, AMP-activated, alpha 2 catalytic subunit

Background

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRKAA2
Entrez
OMIM
Uniprot