Als2 Antibody

Immunohistochemistry: Als2 Antibody [NB100-885] - IHC staining of Als2 in paraffin embedded Human Cortex using NB100-885 at 3.8ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Als2 Antibody Summary

• Description: Novus Biologicals Goat Als2 Antibody (NB100-885) is a polyclonal antibody validated for use in IHC, ELISA and ICC/IF. Anti-Als2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence LKACYYQIQREKLN corresponding to C-Terminus according to NP_065970.2.
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ALS2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:8000
• Application Notes: WB: No signal obtained yet but low background observed in human brain extracts at upto 1 ug/ml. IHC-P: Human cortex shows pixulate cytoplasm staining in some neuronal cells.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22982304).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Als2 Antibody

• ALS2CR6
• alsin
• ALSJ
• amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 6
• amyotrophic lateral sclerosis 2 (juvenile)
• Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 6 protein
• Amyotrophic lateral sclerosis 2 protein
• FLJ31851
• IAHSP
• KIAA1563
• MGC87187
• PLSJ

Background

Defects in ALS2, or Alsin, are the cause of amyotrophic lateral sclerosis 2 (ALS2), juvenile primary lateral sclerosis (JPLS), and infantile-onset ascending spastic paralysis (IAHSP). ALS2 is a neurodegenerative disorder which is closely related to but clinically distinct from juvenile primary lateral sclerosis. It is a progressive paralytic disorder which results from dysfunction of the motor systems comprising the upper motor neurons of the motor cortex and lower motor neurons of the brain stem and spinal cord. JPLS is a neurodegenerative disorder which is closely related to but clinically distinct from amyotrophic lateral sclerosis. It is a progressive paralytic disorder which results from dysfunction of the upper motor neurons of the motor cortex while the lower neurons are unaffected. IAHSP is characterized by progressive spasticity and weakness of limbs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Als2 Antibody (NB100-885)(2)

Publications using NB100-885

• Cobanoglu G, Ozansoy M, Nazl? Ba?ak A. Are alsin and spartin novel interaction partners? Biochem Biophys Res Commun 2012-09-07 [PMID: 22982304] (ICC/IF, Mouse)
• Yang Y, Hentati A, Deng HX et al. The gene encoding alsin, a protein with three guanine-nucleotide exchange factor domains, is mutated in a form of recessive amyotrophic lateral sclerosis. Nat Genet 2001-10-01 [PMID: 11586297]

Bioinformatics

• Gene Symbol: ALS2
• Entrez:
  • 57679(Human)
• Uniprot:
  • Q96Q42(Human)