alpha Tubulin Antibody (YOL1/34) [Alexa Fluor® 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Dr, Fu, YeSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr
Clone
YOL1/34
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 405

alpha Tubulin Antibody (YOL1/34) [Alexa Fluor® 405] Summary

Immunogen
This alpha Tubulin Antibody (YOL1/34) was made from yeast tubulin.
Marker
Microtubule Marker
Specificity
This alpha Tubulin Antibody (YOL1/34) detects an epitope on alpha tubulin between the amino acids 414-422. It has a higher affinity for fixed microtubules (formaldehyde or glutaraldehyde) than native microtubules when performing IHC.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
TUBA1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Flow (Intracellular)

Reactivity Notes

Cross reacts with Arabidopsis, Saccharomyces, Platyzoma, Ashbya, and Naegleria. Based on sequence similarity, is expected to react with: Birds and Mammals.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for alpha Tubulin Antibody (YOL1/34) [Alexa Fluor® 405]

  • alpha Tubulin
  • Alpha-Tubulin 3
  • B-ALPHA-1
  • FLJ25113
  • hum-a-tub1
  • hum-a-tub2
  • LIS3
  • TUBA1A
  • TUBA3
  • tublin
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-Alpha-1
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific

Background

Tubulin is the major building block of microtubules. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. The tubulin superfamily is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa.

Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).

References

1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T.,... Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921

2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha Tubulin Antibody (NB100-1639AF405) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB100-1639AF405
Alexa Fluor 488 NB100-1639AF488
Alexa Fluor 647 NB100-1639AF647
Alexa Fluor 700 NB100-1639AF700
Biotin NB100-1639B
DyLight 350 NB100-1639UV
DyLight 405 NB100-1639V
DyLight 488 NB100-1639G
DyLight 550 NB100-1639R
DyLight 650 NB100-1639C
DyLight 680 NB100-1639FR
DyLight 755 NB100-1639IR
FITC NB100-1639F
HRP NB100-1639H

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Bioinformatics

Gene Symbol TUBA1A