Recombinant Human alpha-Smooth Muscle Actin Protein (NBP2-22670)

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SDS-Page: Recombinant Human alpha-Smooth Muscle Actin Protein [NBP2-22670]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human alpha-Smooth Muscle Actin Protein Summary

Description
A recombinant protein with a His tag corresponding to amino acids 3 - 377 of ACTA2.

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMEEEDS TALVCDNGSG LCKAGFAGDD APRAVFPSIV GRPRHQGVMV GMGQKDSYVG DEAQSKRGIL TLKYPIEHGI ITNWDDMEKI WHHSFYNELR VAPEEHPTLL TEAPLNPKAN REKMTQIMFE TFNVPAMYVA IQAVLSLYAS GRTTGIVLDS GDGVTHNVPI YEGYALPHAI MRLDLAGRDL TDYLMKILTE RGYSFVTTAE REIVRDIKEK LCYVALDFEN EMATAASSSS LEKSYELPDG QVITIGNERF RCPETLFQPS FIGMESAGIH ETTYNSIMKC DIDIRKDLYA NNVLSGGTTM YPGIADRMQK EITALAPSTM KIKIIAPPER KYSVWIGGSI LASLSTFQQM WISKQEYDEA GPSIVHRKCF

Protein/Peptide Type
Recombinant Protein
Gene
ACTA2
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
44.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human alpha-Smooth Muscle Actin Protein

  • AAT6
  • ACTA2
  • Actin alpha 2
  • actin, alpha 2, smooth muscle, aorta
  • actin, aortic smooth muscle
  • ACTSA
  • ACTVS
  • alpha 2 actin
  • alpha SMA
  • alpha smooth muscle actin
  • alpha-actin-2
  • alpha-SMA
  • alphaSmooth Muscle Actin
  • alpha-Smooth Muscle Actin
  • a-sma
  • Cell growth-inhibiting gene 46 protein
  • growth-inhibiting gene 46
  • MYMY5
  • SMA
  • smooth muscle actin

Background

ACTA2 belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. Recombinant human ACTA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Blogs on alpha-Smooth Muscle Actin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Understanding Actin Alpha 2 Smooth Muscle
Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

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Bioinformatics

Gene Symbol ACTA2
Entrez

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