alpha-Smooth Muscle Actin Antibody

Images

 
Western Blot: alpha-Smooth Muscle Actin Antibody [NB300-978] - Staining of Human Duodenum lysate with antibody at 1 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody [NB300-978] - SMA antibody staining on mouse fibroblasts. Primary antibody at 1:1000, incubation at 4C overnight. ICC/IF image submitted by a ...read more
Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody [NB300-978] - Infarcted mouse heart frozen tissue section immunostained with alpha-Smooth Muscle Actin Antibody. IHC-Fr image submitted by a verified ...read more
Western Blot: alpha-Smooth Muscle Actin Antibody [NB300-978] - Staining of HeLa (A) and NIH3T3 (B) cell lysate with antibody at 1 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rb, Rt, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-FrFl
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

alpha-Smooth Muscle Actin Antibody Summary

Immunogen
Alpha-Smooth Muscle Actin Antibody is made to a peptide with sequence EEEDSTALVC corresponding to N-Terminus according to NP_001604.1, NP_001135417.1.
Marker
Mesenchymal Cell Marker
Specificity
Variants NP_001604.1 and NP_001135417.1 encode the same protein.
Predicted Species
Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACTA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 1 ug/mL
Application Notes
This alpha-Smooth Muscle Actin Antibody is validated for IHC-Fr from a verified customer review.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
alpha-Smooth Muscle Actin Overexpression Lysate
Reviewed Applications
Read 7 Reviews rated 5
using
NB300-978 in the following applications:

Publications
Read Publications using
NB300-978 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25619662). Rabbit reactivity reported in scientific literature (PMID: 30480706 and PMID: 33263285).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alpha-Smooth Muscle Actin Antibody

  • AAT6
  • ACTA2
  • Actin alpha 2
  • actin, alpha 2, smooth muscle, aorta
  • actin, aortic smooth muscle
  • ACTSA
  • ACTVS
  • alpha 2 actin
  • alpha SMA
  • alpha smooth muscle actin
  • alpha-actin-2
  • alpha-SMA
  • alphaSmooth Muscle Actin
  • alpha-Smooth Muscle Actin
  • a-sma
  • Cell growth-inhibiting gene 46 protein
  • growth-inhibiting gene 46
  • MYMY5
  • SMA
  • smooth muscle actin

Background

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-223
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-91258
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
AF3844
Species: Hu, Mu
Applications: IHC
NB600-1071
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
7268-CT
Species: Hu
Applications: BA
NBP2-45041
Species: Hu, Po
Applications: Flow, ICC/IF, IF
AF748
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
NBP1-47842
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP2-67327
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
AF3797
Species: Hu, Mu
Applications: ChIP, ICC, IHC, Simple Western, WB
233-FB/CF
Species: Hu
Applications: BA
NBP1-69368
Species: Hu
Applications: IHC, IHC-P, WB
AF3025
Species: Hu
Applications: ELISA, ICC, WB
NBP2-37578
Species: Hu
Applications: ELISA, Flow, ICC/IF, WB
AF3628
Species: Mu, Rt
Applications: CyTOF-ready, Flow, ICC, IHC, WB

Publications for alpha-Smooth Muscle Actin Antibody (NB300-978)(30)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-FrFl, IHC-P.


Filter By Application
ICC/IF
(3)
IHC
(7)
IHC-Fr
(2)
IHC-FrFl
(3)
IHC-P
(5)
All Applications
Filter By Species
Human
(4)
Mouse
(11)
Rabbit
(5)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NB300-978 Applications Species
Wang H, Hironaka C, Mullis D Et al. A Neonatal Leporine Model of Age-Dependent Natural Heart Regeneration After Myocardial Infarction The Journal of Thoracic and Cardiovascular Surgery Aug 1 2021 [PMID: 34649718]
Kim D, Yoon YJ, Choi D Et al. 3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis Frontiers in cell and developmental biology Aug 12 2021 [PMID: 34458260] (IHC-P, Mouse) IHC-P Mouse
Weder B, Schefer F, van Haaften WT Et al. New Therapeutic Approach for Intestinal Fibrosis Through Inhibition of pH-Sensing Receptor GPR4 Inflammatory bowel diseases Jul 28 2021 [PMID: 34320209]
Beylina A, Langston R, Rosen D et al. Generation of fourteen isogenic cell lines for Parkinsons disease-associated leucine-rich repeat kinase (LRRK2) Stem Cell Research May 1 2021 [PMID: 34087985] (ICC/IF, Human) ICC/IF Human
Yildirim M, Oztay F, Kayalar O, Tasci AE Effect of long noncoding RNAs on epithelial-mesenchymal transition in A549 cells and fibrotic human lungs Journal of cellular biochemistry Apr 13 2021 [PMID: 33847014]
Huang N, PErez P, Kato T et al. SARS-CoV-2 infection of the oral cavity and saliva Nature medicine Mar 25 2021 [PMID: 33767405] (IHC-P) IHC-P
Yang Z, Wang W, Zhao L et al. Plasma cells shape the mesenchymal identity of ovarian cancers through transfer of exosome-derived microRNAs Science advances Feb 1 2021 [PMID: 33627414]
Kirst C, Skriabine S, Vieites-Prado A et al. Mapping the Fine-Scale Organization and Plasticity of the Brain Vasculature Cell Feb 20 2020 [PMID: 32059781] (IHC, Mouse) IHC Mouse
Geng X, Xu Z, Tu C et al. Hydrogel Complex Electrospun Scaffolds and Their Multiple Functions in In Situ Vascular Tissue Engineering ACS Applied Bio Materials Feb 9 2021
Carlos de Oliveira R, Tye G, Sampaio L et al. TGF beta 1 and TGF beta 2 proteins in corneas with and without stromal fibrosis: Delayed regeneration of apical epithelial growth factor barrier and the epithelial basement membrane in corneas with stromal fibrosis Experimental Eye Research Oct 1 2020 [PMID: 33263285] (IHC-Fr, Rabbit) IHC-Fr Rabbit
Show All 30 Publications.

Reviews for alpha-Smooth Muscle Actin Antibody (NB300-978) (7) 57

Average Rating: 5
(Based on 7 reviews)
We have 7 reviews tested in 3 species: Human, Mouse, Rabbit.

Reviews using NB300-978:
Filter by Applications
ICC
(4)
WB
(1)
IHC-Fr
(2)
All Applications
Filter by Species
Human
(2)
Mouse
(3)
Rabbit
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry alpha-Smooth Muscle Actin NB300-978
Enlarge
5
reviewed by:
Verified Customer
ICC Mouse 09/15/2021
View

Summary

ApplicationImmunocytochemistry
Sample Tested fibroblasts
SpeciesMouse
LotS4C2G2P26

Comments

CommentsPrimary antibody 1:1000 concentration, incubation overnight at 4C.
Western Blot alpha-Smooth Muscle Actin NB300-978
Enlarge
5
reviewed by:
Verified Customer
WB Human 03/31/2021
View

Summary

ApplicationWestern Blot
Sample Tested fibroblasts
SpeciesHuman
LotS4C2G2P27
Immunocytochemistry alpha-Smooth Muscle Actin NB300-978
Enlarge
5
reviewed by:
Verified Customer
ICC Mouse 01/14/2021
View

Summary

ApplicationImmunocytochemistry
Sample TestedAdult testis
SpeciesMouse
LotS4C4P7E191118

Comments

Commentsprimary antibody 1:200 dilution.
Immunohistochemistry-Frozen alpha-Smooth Muscle Actin NB300-978
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 01/30/2020
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse heart frozen sections
SpeciesMouse
LotS4C2Q2P26

Comments

CommentsMouse frozen heart after myocardial infarction
PFA fixed
Primary antibody 1:200
Secondary antibody 1:500
Immunocytochemistry alpha-Smooth Muscle Actin NB300-978
Enlarge
5
reviewed by:
Verified Customer
ICC Human 10/09/2019
View

Summary

ApplicationImmunocytochemistry
Sample Tested fibroblasts
SpeciesHuman
LotS4C4P7E191118
  5
reviewed by:
Paramananda Saikia
IHC-Fr Rabbit 10/23/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedcorneal stromal cells
SpeciesRabbit
LotS4C4P6E060818
  5
reviewed by:
Paramananda Saikia
ICC Rabbit 05/31/2018
View

Summary

ApplicationImmunocytochemistry
Sample TestedEye lens tissue
SpeciesRabbit
LotS464P3N24051

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha-Smooth Muscle Actin Antibody (NB300-978). (Showing 1 - 2 of 2 FAQs).

  1. I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?
    • We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.
  2. Which antibodies would you recommend for the best performance in IHC?
    • NB300-978, NBP1-30894, NBP2-22120, NBP2-32808, NBP2-33006, and NBP2-34522 have the broadest IHC applications, as they additionally list IHC-Paraffin and IHC-Frozen. To narrow the list down further, we would recommend using either NB300-978 or NBP2-33006 since these products have the most ratings and cited publications for use in IHC methods. NB300-978 has IHC-Free-Floating listed as an application as well.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional alpha-Smooth Muscle Actin Products

Bioinformatics Tool for alpha-Smooth Muscle Actin Antibody (NB300-978)

Discover related pathways, diseases and genes to alpha-Smooth Muscle Actin Antibody (NB300-978). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for alpha-Smooth Muscle Actin Antibody (NB300-978)

Discover more about diseases related to alpha-Smooth Muscle Actin Antibody (NB300-978).
 

Pathways for alpha-Smooth Muscle Actin Antibody (NB300-978)

View related products by pathway.

PTMs for alpha-Smooth Muscle Actin Antibody (NB300-978)

Learn more about PTMs related to alpha-Smooth Muscle Actin Antibody (NB300-978).
 

Research Areas for alpha-Smooth Muscle Actin Antibody (NB300-978)

Find related products by research area.

Blogs on alpha-Smooth Muscle Actin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Understanding Actin Alpha 2 Smooth Muscle
Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
7
4
0
3
0
2
0
1
0

Verified Customer
09/15/2021
Application: ICC
Species: Mouse

Verified Customer
03/31/2021
Application: WB
Species: Human

Verified Customer
01/14/2021
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol ACTA2
Entrez
Uniprot