alpha-Smooth Muscle Actin Overexpression Lysate (Native)

Images

 
Western Blot: Actin Alpha 2 Smooth Muscle Overexpression Lysate (Adult Normal) [NBP2-11075] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Actin Alpha 2 Smooth ...read more

Product Details

Summary
Applications WB

Order Details

alpha-Smooth Muscle Actin Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_001135417. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Actin Alpha 2 Smooth Muscle, mRNA.
Gene
ACTA2

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
41.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for alpha-Smooth Muscle Actin

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for alpha-Smooth Muscle Actin Overexpression Lysate (Native)

  • AAT6
  • ACTA2
  • Actin alpha 2
  • actin, alpha 2, smooth muscle, aorta
  • actin, aortic smooth muscle
  • ACTSA
  • ACTVS
  • alpha 2 actin
  • alpha SMA
  • alpha smooth muscle actin
  • alpha-actin-2
  • alpha-SMA
  • alphaSmooth Muscle Actin
  • alpha-Smooth Muscle Actin
  • a-sma
  • Cell growth-inhibiting gene 46 protein
  • growth-inhibiting gene 46
  • MYMY5
  • SMA
  • smooth muscle actin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for alpha-Smooth Muscle Actin Lysate (NBP2-11075) (0)

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Video Protocols

WB Video Protocol

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Blogs on alpha-Smooth Muscle Actin.

Understanding Actin Alpha 2 Smooth Muscle
Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol ACTA2
Entrez

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