alpha-Smooth Muscle Actin Antibody (SPM332)

Images

 
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - Analysis using the Azide and BSA Free version of NBP2-32808. Staining of Leiomyosarcoma.
Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PE (blue) and a matched ...read more
Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760APC (blue) and a matched ...read more
Flow (Intracellular): alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HeLa cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760PCP (blue) and a matched ...read more
Flow Cytometry: alpha-Smooth Muscle Actin Antibody (SPM332) [NBP2-32808] - An intracellular stain was performed on HepG2 cells with alpha-Smooth Muscle Actin Antibody (SPM332) NBP2-34760AF647 (blue) and a matched ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bb, Bv, Ca, Ch, Fe, GP, Gt, Pm, Rb, ShSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC-Fr, IHC-P, IP
Clone
SPM332
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

alpha-Smooth Muscle Actin Antibody (SPM332) Summary

Immunogen
N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH
Localization
Cytoplasmic
Marker
Leiomyosarcoma Marker
Specificity
Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACTA2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
  • Flow (Intracellular)
Application Notes
ELISA (For coating, order NBP2-34760 (BSA free version of NBP2-32808))
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-32808 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS with 0.05% BSA.
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for alpha-Smooth Muscle Actin Antibody (SPM332)

  • AAT6
  • ACTA2
  • Actin alpha 2
  • actin, alpha 2, smooth muscle, aorta
  • actin, aortic smooth muscle
  • ACTSA
  • ACTVS
  • alpha 2 actin
  • alpha SMA
  • alpha smooth muscle actin
  • alpha-actin-2
  • alpha-SMA
  • alphaSmooth Muscle Actin
  • alpha-Smooth Muscle Actin
  • a-sma
  • Cell growth-inhibiting gene 46 protein
  • growth-inhibiting gene 46
  • MYMY5
  • SMA
  • smooth muscle actin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-223
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
Applications: WB, ICC/IF, ICC
NBP1-91258
Species: Hu, Mu, Bv, Ca, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
AF3844
Species: Hu, Mu
Applications: IHC
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-724
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-67327
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
AF3797
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
NBP1-69368
Species: Hu
Applications: WB, IHC, IHC-P
AF3025
Species: Hu
Applications: WB, ELISA, ICC
NBP2-37578
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
AF3628
Species: Mu, Rt
Applications: WB, Flow, IHC, CyTOF-ready, ICC

Publications for alpha-Smooth Muscle Actin Antibody (NBP2-32808)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for alpha-Smooth Muscle Actin Antibody (NBP2-32808) (0)

There are no reviews for alpha-Smooth Muscle Actin Antibody (NBP2-32808). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha-Smooth Muscle Actin Antibody (NBP2-32808) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for alpha-Smooth Muscle Actin Antibody (NBP2-32808)

Discover related pathways, diseases and genes to alpha-Smooth Muscle Actin Antibody (NBP2-32808). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for alpha-Smooth Muscle Actin Antibody (NBP2-32808)

Discover more about diseases related to alpha-Smooth Muscle Actin Antibody (NBP2-32808).
 

Pathways for alpha-Smooth Muscle Actin Antibody (NBP2-32808)

View related products by pathway.

PTMs for alpha-Smooth Muscle Actin Antibody (NBP2-32808)

Learn more about PTMs related to alpha-Smooth Muscle Actin Antibody (NBP2-32808).
 

Research Areas for alpha-Smooth Muscle Actin Antibody (NBP2-32808)

Find related products by research area.

Blogs on alpha-Smooth Muscle Actin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Understanding Actin Alpha 2 Smooth Muscle
Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our alpha-Smooth Muscle Actin Antibody (SPM332) and receive a gift card or discount.

Bioinformatics

Gene Symbol ACTA2
Uniprot