alpha-Smooth Muscle Actin Antibody (132D3) [mFluor Violet 450 SE]

alpha-Smooth Muscle Actin Antibody (132D3) [mFluor Violet 450 SE] [NBP2-59429MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 132D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 450 SE

alpha-Smooth Muscle Actin Antibody (132D3) [mFluor Violet 450 SE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Alpha-Smooth Muscle Actin Antibody (132D3) is made to a recombinant human ACTA2 (3-377aa) purified from E. coli.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-59429. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for alpha-Smooth Muscle Actin Antibody (132D3) [mFluor Violet 450 SE]

• AAT6
• ACTA2
• Actin alpha 2
• actin, alpha 2, smooth muscle, aorta
• actin, aortic smooth muscle
• ACTSA
• ACTVS
• alpha 2 actin
• alpha SMA
• alpha smooth muscle actin
• alpha-actin-2
• alpha-SMA
• alphaSmooth Muscle Actin
• alpha-Smooth Muscle Actin
• a-sma
• Cell growth-inhibiting gene 46 protein
• growth-inhibiting gene 46
• MYMY5
• SMA
• smooth muscle actin

Background

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for alpha-Smooth Muscle Actin Antibody (NBP2-59429MFV450). (Showing 1 - 1 of 1 FAQ).

1. I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?
   • We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.

Bioinformatics

• Gene Symbol: ACTA2