Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free [NBP3-25359] - alpha Actinin 2 antibody [HL1692] detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: alpha Actinin 2 stained by alpha Actinin 2 antibody [HL1692] (NBP3-25359) diluted at 1:500. Blue: Fluoroshield with DAPI .

Western Blot: Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free [NBP3-25359] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin antibody [HL1692] (NBP3-25359) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free [NBP3-25359] - alpha Actinin 2 antibody [HL1692] detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. alpha Actinin 2 stained by alpha Actinin 2 antibody [HL1692] (NBP3-25359) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: HL1692
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free (NBP3-25359) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragmemt of human Alpha Actinin (pan)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACTN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Alpha Actinin (pan) Antibody (HL1692) - Azide and BSA Free

• actinin 1 smooth muscle
• actinin, alpha 1
• alpha-actinin 1
• Alpha-actinin cytoskeletal isoform
• alpha-actinin-1
• F-actin cross-linking protein
• FLJ40884
• FLJ54432
• Non-muscle alpha-actinin-1

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins,including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple rolesin different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles andadherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, andsmooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillaractin filaments. This gene encodes a nonmuscle, cytoskeletal, alp

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTN1