Alpha Actinin 2 Antibody

Images

 
Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Paraffin-embedded Heart, using antibody 10 ug/ml.
Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 (Mouse)
Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Rat tissue extracts (50ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody diluted at 1:5000.
Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects ACTN2 protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extractC. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.390 mg/ml

Order Details

Alpha Actinin 2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.
Localization
Cytoplasm, myofibril, sarcomere, Z-disk
Predicted Species
Porcine (99%), Xenopus (94%), Bovine (99%), Chicken (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACTN2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (94%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.390 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Alpha Actinin 2 Antibody

  • actinin, alpha 2
  • Alpha-actinin skeletal muscle isoform 2
  • alpha-actinin skeletal muscle
  • alpha-actinin-2
  • CMD1AA
  • F-actin cross-linking protein

Background

Alpha actinins are actin binding proteins that carry out different purposes in different different cell types. Skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN2 encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. There are several known transcript variants that result from the use of multiple polyA+ sites. ACTN2 has the ability to form both homo- and heterodimers (with ACTN3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ACTN2