Alpha Actinin 2 Antibody

Images

 
Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] diluted at 1:1000. The ...read more
Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: alpha Actinin 2 protein stained by alpha Actinin 2 antibody [N1N3] diluted at ...read more
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse muscle. alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Rat tissue extracts (50ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody diluted at 1:5000.
Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects ACTN2 protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extractC. ...read more
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Rat muscle. alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse heart. Alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Rat heart, alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse heart. alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ze, Bv, Ch, XpSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Alpha Actinin 2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.
Localization
Cytoplasm, myofibril, sarcomere, Z-disk
Predicted Species
Bovine (99%), Xenopus (94%), Chicken (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACTN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
Theoretical MW
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-32462 in the following applications:

Reactivity Notes

Xenopus laevis (94%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Alpha Actinin 2 Antibody

  • Actinin alpha 2
  • actinin, alpha 2
  • Actinin-a2
  • ACTN2
  • alpha Actinin 2
  • alpha-Actinin 2
  • Alpha-actinin skeletal muscle isoform 2
  • Alpha-Actinin Skeletal Muscle
  • Alpha-Actinin-2
  • CMD1AA
  • F-actin cross-linking protein

Background

Alpha actinins are actin binding proteins that carry out different purposes in different different cell types. Skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN2 encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. There are several known transcript variants that result from the use of multiple polyA+ sites. ACTN2 has the ability to form both homo- and heterodimers (with ACTN3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alpha Actinin 2 Antibody (NBP1-32462)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC/IF, IF/IHC.


Filter By Application
ICC/IF
(1)
IF/IHC
(1)
All Applications
Filter By Species
Mouse
(2)
All Species
Showing Publications 1 - 2 of 2.
Publications using NBP1-32462 Applications Species
Meyers TA, Heitzman JA, Krebsbach AM et al. Acute AT1R blockade prevents isoproterenol-induced injury in mdx hearts. J. Mol. Cell. Cardiol. 2019-01-19 [PMID: 30664850] (ICC/IF, Mouse) ICC/IF Mouse
Meyers TA Preclinical Models of Dystrophic Cardiomyopathy and Therapies for the Dystrophic Heart Thesis (IF/IHC, Mouse) IF/IHC Mouse

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ACTN2