Alpha Actinin 2 Antibody

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse muscle. alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Rat muscle. alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse heart. Alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Rat heart, alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse heart. alpha Actinin 2 stained by alpha Actinin 2 antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Blue: Hoechst 33342 staining.

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Blue: Fluoroshield with DAPI .

Immunocytochemistry/ Immunofluorescence: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Blue: Fluoroshield with DAPI .

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Alpha Actinin 2 Antibody [NBP1-32462] - Alpha Actinin 2 antibody [N1N3] detects Alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.Alpha Actinin 2 stained by Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Alpha Actinin 2 Antibody [NBP1-32462] - Whole zebrafish extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (NBP1-32462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Ze, Bv, Ch, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Alpha Actinin 2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Alpha Actinin 2. The exact sequence is proprietary.
• Localization: Cytoplasm, myofibril, sarcomere, Z-disk
• Predicted Species: Bovine (99%), Xenopus (94%), Chicken (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACTN2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 104 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (94%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Alpha Actinin 2 Antibody

• Actinin alpha 2
• actinin, alpha 2
• Actinin-a2
• ACTN2
• alpha Actinin 2
• alpha-Actinin 2
• Alpha-actinin skeletal muscle isoform 2
• Alpha-Actinin Skeletal Muscle
• Alpha-Actinin-2
• CMD1AA
• F-actin cross-linking protein

Background

Alpha actinins are actin binding proteins that carry out different purposes in different different cell types. Skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN2 encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. There are several known transcript variants that result from the use of multiple polyA+ sites. ACTN2 has the ability to form both homo- and heterodimers (with ACTN3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alpha Actinin 2 Antibody (NBP1-32462)(2)

Publications using NBP1-32462

• Meyers TA, Heitzman JA, Krebsbach AM et al. Acute AT1R blockade prevents isoproterenol-induced injury in mdx hearts. J. Mol. Cell. Cardiol. 2019-01-19 [PMID: 30664850] (ICC/IF, Mouse)
• Meyers TA Preclinical Models of Dystrophic Cardiomyopathy and Therapies for the Dystrophic Heart Thesis (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: ACTN2