PDLIM4 Antibody

Western Blot: PDLIM4 Antibody [NBP1-80833] - SH-I-14 induces demethylation and re-expression of tumor suppressor genes. Cells were treated with SH-I-14 for 48 hr and subjected to western blot analysis. The lysates from the cells treated with SH-I-14 for 48 hr were analyzed by western blot with indicated antibodies. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/4054), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NBP1-80833] - Staining of human cell line U-2 OS shows localization to actin filaments. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human small intestine shows weak to moderate cytoplasmic positivity in glandular cells.

Western Blot: PDLIM4 Antibody [NBP1-80833] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Western Blot: PDLIM4 Antibody [NBP1-80833] - Analysis in human cell line RH-30.

Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human Fallopian tube shows weak to moderate membranous and cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.

• Reactivity: Hu, Rt, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PDLIM4 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: HSVTLRGPSPWGFRLVGGRDFSAPLTISRVHAGSKAALAALCPGDLIQAINGESTELMTHLEAQNRIKGCHDHLTLSVSRPEGRSWPSAPDDSKAQAHRIHIDPE
• Predicted Species: Mouse (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDLIM4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: WB reported in scientific literature (PMID: 29137356). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PDLIM4 Recombinant Protein Antigen (NBP1-80833PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PDLIM4 Antibody

• LIM domain protein
• LIM protein RIL
• PDZ and LIM domain 4
• PDZ and LIM domain protein 4
• Reversion-induced LIM protein
• RILenigma homolog

Background

PDLIM4, also known as PDZ and LIM domain protein 4, has 2 isoforms, a 330 amino acid long isoform that is 35 kDa and a short 246 amino acid isoform that is 26 kDa and found in brain. May be involved in bone development. Disease research is being performed on this protein involvement in osteoporosis, bronchiolitis, prostate cancer, prostatitis, multiple personality disorder, and asperger syndrome. The protein interacts with RBPMS, PTPN13, TRIP6, MYC, ZNF408, ABCA1, NFE2, SLC2A4, SSBP, TCF3, and ZBTB9 proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDLIM4 Antibody (NBP1-80833)(4)

Publications using NBP1-80833

• Xiao Q, Kang Z, Liu C et al. Panax notoginseng Saponins Attenuate Cerebral Ischemia-Reperfusion Injury via Mitophagy-Induced Inhibition of NLRP3 Inflammasome in Rats Frontiers in Bioscience-Landmark 2022-11-03 [PMID: 36472098] (WB, Rat)
  
  Details:
  Dilution used 1:500
• Kang HJ, Yi YW, Hou SJ et al. Disruption of STAT3-DNMT1 interaction by SH-I-14 induces re-expression of tumor suppressor genes and inhibits growth of triple-negative breast tumor Oncotarget. 2017-10-13 [PMID: 29137356] (WB, Human)
• de Tayrac M, Saikali S, Aubry M et al. Prognostic Significance of EDN/RB, HJURP, p60/CAF-1 and PDLI4, Four New Markers in High-Grade Gliomas. PLoS One 2013-01-01 [PMID: 24039914]
• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]

Bioinformatics

• Gene Symbol: PDLIM4
• Entrez:
  • 8572(Human)
• Uniprot:
  • P50479(Human)