Alpha Actinin 4 Antibody

Images

 
Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. ...read more
Immunocytochemistry/ Immunofluorescence: Alpha Actinin 4 Antibody [NBP2-15357] - Paraformaldehyde-fixed HeLa, using alpha Actinin 4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha ...read more
Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP2-15357] - Paraffin-embedded mouse intestine alpha Actinin 4 antibody, C-term dilution: 1:500.
Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - 30 ug PC-12 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.
Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP2-15357] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.32 mg/ml

Order Details

Alpha Actinin 4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human alpha Actinin 4. The exact sequence is proprietary.
Localization
Nucleus; Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACTN4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.32 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Alpha Actinin 4 Antibody

  • actinin alpha4 isoform
  • actinin, alpha 4
  • ACTININ-4
  • alpha-actinin-4
  • DKFZp686K23158
  • F-actin cross-linking protein
  • focal segmental glomerulosclerosis 1
  • FSGS
  • FSGS1
  • Non-muscle alpha-actinin 4

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ACTN4
Uniprot