Western Blot: alpha-Actinin 1 Antibody [NBP1-85791] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate ...read more
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-85791] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Western Blot: alpha-Actinin 1 Antibody [NBP1-85791] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). ...read more
This antibody was developed against Recombinant Protein corresponding to amino acids:SAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKV
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Immunogen affinity purified
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Similarly to my findings when probing against actin, in multiple species (mouse and dog), double banding appears at in tissues dense with actin. I've found that, when probing against actin, decreasing primary antibody concentration in these samples reduces, and can eliminate the non-specific band. Took a lot of troubleshooting to find the sweet spot, and I have yet to go through that process with this alpha actinin antibody.
I have found that anti-actin and actinin antibodies tend to produce a double band, especially in tissues where actin is present in high amounts. I've found that decreasing primary antibody concentration when probing for actin (1:10000), reduces this background band, but have yet to test that for this antibody.
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FAQs for alpha-Actinin 1 Antibody (NBP1-85791). (Showing 1 - 1 of 1 FAQ).
Is this item restricted under "Cartagena Law"?
To our knowledge this item is not restricted under the Cartagena Protocol, but this antibody is RUO. We have not tested or certified this product for use in vivo or for diagnostic use. Any use of this kind is undertaken by the end user at their own risk.
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