alpha-Actinin 1 Antibodies
We offer Alpha Actinin 1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Western Blot. Each Alpha Actinin 1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Alpha Actinin 1 Antibodies can be used in a variety of model species: Canine, Human, Mouse, Rat. Use the list below to choose the Alpha Actinin 1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Alpha Actinin 1 polyclonal antibodies and browse our Alpha Actinin 1 monoclonal antibody catalog.
Alternate Names for alpha-Actinin 1 Antibodies
anti-alpha-Actinin 1 antibody, anti-ACTN1 antibody, anti-actinin 1 smooth muscle antibody, anti-actinin, alpha 1 antibody, anti-Alpha-actinin cytoskeletal isoform antibody, anti-alpha-actinin-1 antibody, anti-F-actin cross-linking protein antibody, anti-FLJ40884 antibody, anti-FLJ54432 antibody, anti-Non-muscle alpha-actinin-1 antibody
10 Results for "alpha-actinin-1" in Primary Antibodies
![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ACTN1 monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Western-Blot-NBP1-48251-img0020.jpg "Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ACTN1 monoclonal antibody (1:200).")
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of Hela cells transiently transfected by pCMV6-ENTRY Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunocytochemistry-Immunofluorescence-NBP1-48251-img0011.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Ovary tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0016.jpg)
![Western Blot: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Western-Blot-NBP1-48251-img0018.jpg)
![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from 8 different cell lines by using anti-ACTN1 monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Western-Blot-NBP1-48251-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of HeLa cells using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunocytochemistry-Immunofluorescence-NBP1-48251-img0010.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human bladder tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0012.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human breast tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0013.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Kidney tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0014.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human liver tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0015.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, and ACTN1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ACTN1 monoclonal antibody (1:500`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Knockout-Validated-NBP1-48251-img0021.jpg)
![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Western-Blot-NBP2-70078-img0002.jpg "Western Blot: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha")
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of HeLa cells using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70078-img0004.jpg)
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human liver tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0008.jpg)
![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Alpha Actinin 1 antibody (7A4) at 1:2000 + recombinant human Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Western-Blot-NBP2-70078-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of Hela cells transiently transfected by pCMV6-ENTRY Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70078-img0003.jpg)
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human breast tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0005.jpg)
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human Kidney tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0006.jpg)
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human bladder tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0007.jpg)
![Immunohistochemistry: alpha-Actinin 1 Antibody (OTI7A4) - Azide and BSA Free [NBP2-70078] - Staining of paraffin-embedded Human Ovary tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70078-img0009.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-85791] - Staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85791-img0010.jpg "Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-85791] - Staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.")
![Western Blot: alpha-Actinin 1 Antibody [NBP1-85791] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-85791-img0005.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-32578-img0013.jpg "Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-32578] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-32578-img0007.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - A:NIH-3T3.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-32578-img0008.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-32578-img0011.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-32578-img0012.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Whole zebrafish extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody [N2N3] (NBP1-32578) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-32578_rabbit-polyclonal-alpha-actinin-1-antibody-91020242063315.jpg)
![Immunohistochemistry: alpha-Actinin 1 Antibody [NBP1-32578] - alpha-Actinin 1 antibody [N2N3] detects alpha-Actinin 1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. alpha-Actinin 1 antibody [N2N3] (NBP1-32578) dilution: 1:50.](http://images.novusbio.com/fullsize/nbp1-32578_rabbit-polyclonal-alpha-actinin-1-antibody-91020242091449.jpg)
![Western Blot: alpha-Actinin 1 Antibody (3F1) [H00000087-M01] - Analysis of ACTN1 expression in transfected 293T cell line by ACTN1 monoclonal antibody (M01), clone 3F1.Lane 1: ACTN1 transfected lysate(103.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-3F1-Western-Blot-H00000087-M01-img0002.jpg "Western Blot: alpha-Actinin 1 Antibody (3F1) [H00000087-M01] - Analysis of ACTN1 expression in transfected 293T cell line by ACTN1 monoclonal antibody (M01), clone 3F1.Lane 1: ACTN1 transfected lysate(103.1 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: alpha-Actinin 1 Antibody (A1G2-R) [NBP3-32030] - Western blot analysis of alpha-Actinin 1 on different lysates with Mouse anti-alpha-Actinin 1 antibody (NBP3-32030) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 103 kDa Observed band size: 103 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32030) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32030_mouse-alpha-actinin-1-mab-a1g2-r-27820241514175.jpg "Western Blot: alpha-Actinin 1 Antibody (A1G2-R) [NBP3-32030] - Western blot analysis of alpha-Actinin 1 on different lysates with Mouse anti-alpha-Actinin 1 antibody (NBP3-32030) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 103 kDa Observed band size: 103 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32030) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha-Actinin 1 Antibody (A1G2-R) [NBP3-32030] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-alpha-Actinin 1 antibody (NBP3-32030) at 1/6,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32030) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32030_mouse-alpha-actinin-1-mab-a1g2-r-298202414304181.jpg)
![Western Blot: alpha-Actinin 1 Antibody (1D10) [NBP2-59465] - Lane 1: PC-3 cell lysates, Lane 2: Balb/3T3 cell lysates, Lane 3: A549 cell lysates](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-1D10-Western-Blot-NBP2-59465-img0001.jpg "Western Blot: alpha-Actinin 1 Antibody (1D10) [NBP2-59465] - Lane 1: PC-3 cell lysates, Lane 2: Balb/3T3 cell lysates, Lane 3: A549 cell lysates")