alpha-Actinin 1 Antibody

Images

 
Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody diluted at a dilution of 1:1000.
Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-32578] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.
Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - A:NIH-3T3.
Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - A: 293TB: A431 C: H1299D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.09 mg/ml

Order Details

alpha-Actinin 1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Actinin 1. The exact sequence is proprietary.
Localization
Cytoplasm, cytoskeleton, myofibril, sarcomere, Z-disk
Marker
Z-disk Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACTN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-32578 in the following applications:

  • WB
    1 publication

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (97%) and Xenopus tropicalis (97%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.09 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alpha-Actinin 1 Antibody

  • actinin 1 smooth muscle
  • actinin, alpha 1
  • ACTN1
  • alphaActinin 1
  • alpha-Actinin 1
  • Alpha-actinin cytoskeletal isoform
  • alpha-actinin-1
  • BDPLT15
  • F-actin cross-linking protein
  • FLJ40884
  • FLJ54432
  • Non-muscle alpha-actinin-1

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Actinin 1 Antibody (NBP1-32578)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ACTN1
Entrez