alpha-Actinin 1 Antibody - BSA Free

Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-32578] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.

Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - A:NIH-3T3.

Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody diluted at a dilution of 1:1000.

Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Whole zebrafish extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody [N2N3] (NBP1-32578) diluted at 1:1000.

Immunohistochemistry: alpha-Actinin 1 Antibody [NBP1-32578] - alpha-Actinin 1 antibody [N2N3] detects alpha-Actinin 1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. alpha-Actinin 1 antibody [N2N3] (NBP1-32578) dilution: 1:50.

• Reactivity: Hu, Mu, Rt, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

alpha-Actinin 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit alpha-Actinin 1 Antibody - BSA Free (NBP1-32578) is a polyclonal antibody validated for use in IHC and WB. Anti-alpha-Actinin 1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha-Actinin 1. The exact sequence is proprietary.
• Localization: Cytoplasm, cytoskeleton, myofibril, sarcomere, Z-disk
• Marker: Z-disk Marker
• Predicted Species: Xenopus (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACTN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 103 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for alpha-Actinin 1 Antibody - BSA Free

• actinin 1 smooth muscle
• actinin, alpha 1
• ACTN1
• alpha Actinin 1
• alphaActinin 1
• alpha-Actinin 1
• Alpha-actinin cytoskeletal isoform
• alpha-actinin-1
• BDPLT15
• F-actin cross-linking protein
• FLJ40884
• FLJ54432
• Non-muscle alpha-actinin-1

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for alpha-Actinin 1 Antibody (NBP1-32578)(1)

Publication using NBP1-32578

• Hosseinibarkooie S, Peters M, Benito LT et al. The Power of Human Protective Modifiers: PLS3 and CORO1C Unravel Impaired Endocytosis in Spinal Muscular Atrophy and Rescue SMA Phenotype. Am J Hum Genet. 2016-08-04 [PMID: 27499521] (WB, Mouse)

Bioinformatics

• Gene Symbol: ACTN1