alpha 2-Macroglobulin Antibody (NB600-938)

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Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1,000 overnight at 4C. Secondary ...read more
Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ug per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4C. Secondary ...read more
Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Analysis using the Biotin conjugate of NB600-938. Detection of Lane 1: alpha 2-Macroglobulin Antibody. Lane 2: none. Load: 100 ug per lane. Primary ...read more
Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Detection of Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were ...read more
Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Analysis of Biotin conjugate of NB600-938. Goat anti Alpha-2-Macroglobulin antibody was used to detect alpha 2-Macroglobulin under reducing (R) and non-reducing ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price

alpha 2-Macroglobulin Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Alpha-2-Macroglobulin [Human Plasma]
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
A2M
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:20000
  • ELISA 1:80000-1:400000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for alpha 2-Macroglobulin Antibody

  • A2M
  • alpha 2Macroglobulin
  • alpha 2-Macroglobulin
  • alpha-2-M
  • alpha-2-macroglobulin
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
  • CPAMD5
  • CPAMD5DKFZp779B086
  • FWP007
  • S863-7

Background

Alpha 2 macroglobulin is a serum protein which is mainly synthesised in the liver. It functions as a broad range irreversible proteinase inhibitor that forms a "trap" around most proteases. Alpha 2 macroglobulin is is a tetrameric molecule, and with a molecular weight of 720 kDa, it so large a molecule that it tends to remain intravascular. Following conformational change, the protinase is deposited within a central cavity where it is still active but prevented from further contact with its substrate. Removal of the complex is mediated by the generation of recognition sites which react with receptors on a variety of cells including macrophages and liver cells. Alpha 2 macroglobulin is localized on the surface of peripheral blood lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol A2M
Entrez
Uniprot