alpha 2-Macroglobulin Antibody (A2M/3623) - Azide and BSA Free

Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-08689] - Formalin-fixed, paraffin-embedded human liver stained with alpha 2-Macroglobulin Mouse Monoclonal Antibody (A2M/3623).

Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-08689] - Formalin-fixed, paraffin-embedded human liver stained with alpha 2-Macroglobulin Mouse Monoclonal Antibody (A2M/3623).

Protein Array: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-08689] - Analysis of Protein Array containing more than 19,000 full-length human proteins using alpha 2-Macroglobulin Mouse Monoclonal Antibody (A2M/3623).

• Reactivity: Hu
• Applications: IHC, MA
• Clone: A2M/3623
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

alpha 2-Macroglobulin Antibody (A2M/3623) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07710).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human recombinant alpha 2-Macroglobulin protein fragment (around aa 604-748) (exact sequence is proprietary) (Uniprot: P01023)
• Localization: Secreted in plasma
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: A2M
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
• Application Notes: Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
• Theoretical MW: 185 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for alpha 2-Macroglobulin Antibody (A2M/3623) - Azide and BSA Free

• A2M
• alpha 2Macroglobulin
• alpha 2-Macroglobulin
• Alpha-2-M
• alpha-2-macroglobulin
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
• CPAMD5
• CPAMD5DKFZp779B086
• FWP007
• S863-7

Background

Alpha 2 macroglobulin is a serum protein which is mainly synthesised in the liver. It functions as a broad range irreversible proteinase inhibitor that forms a "trap" around most proteases. Alpha 2 macroglobulin is is a tetrameric molecule, and with a molecular weight of 720 kDa, it so large a molecule that it tends to remain intravascular. Following conformational change, the protinase is deposited within a central cavity where it is still active but prevented from further contact with its substrate. Removal of the complex is mediated by the generation of recognition sites which react with receptors on a variety of cells including macrophages and liver cells. Alpha 2 macroglobulin is localized on the surface of peripheral blood lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: A2M