alpha 2-Macroglobulin Antibody (257303) [Unconjugated]

Recombinant Human alpha 2‑Macroglobulin protein was serially diluted 2-fold and captured by Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # MAB19381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human alpha 2‑Macroglobulin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1938) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: ELISA
• Clone: 257303
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

alpha 2-Macroglobulin Antibody (257303) [Unconjugated] Summary

• Immunogen: Human plasma-derived alpha ₂-Macroglobulin
• Specificity: Detects human alpha ₂-Macroglobulin in direct ELISA, this antibody does not cross-react with rhC5a or rmC5a.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha 2-Macroglobulin Antibody (257303) [Unconjugated]

• A2M
• alpha 2Macroglobulin
• alpha 2-Macroglobulin
• alpha-2-M
• alpha-2-macroglobulin
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
• CPAMD5
• CPAMD5DKFZp779B086
• FWP007
• S863-7

Background

Alpha 2-macroglobulin (A2M) is a general and irreversible protease inhibitor implicated in many processes. It is able to inhibit all four classes of proteases by a unique trapping mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.