Aldolase A Antibody (1C5B2) - BSA Free

Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mAb against HEK293 (1) and ALDOA (AA: 9-145)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis of breast cancer tissues using ALDOA mouse mAb with DAB staining.

Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mAb against human ALDOA (AA: 9-145) recombinant protein. (Expected MW is 40.7 kDa)

Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mouse mAb against MCF-7 (1), Hela (2), and NIH/3T3 (3) cell lysate.

Immunohistochemistry-Paraffin: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis of bladder cancer tissues using ALDOA mouse mAb with DAB staining.

ELISA: Aldolase A Antibody (1C5B2) [NBP2-61844] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

ELISA: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis of K562 cells using ALDOA mouse mAb (green) and negative control (red).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, IHC
• Clone: 1C5B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Aldolase A Antibody (1C5B2) - BSA Free Summary

• Description: Novus Biologicals Mouse Aldolase A Antibody (1C5B2) - BSA Free (NBP2-61844) is a monoclonal antibody validated for use in IHC, WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Aldolase A (AA: 9-145) expressed in E. Coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDOA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:100-1:2000
• Theoretical MW: 39.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Aldolase A Antibody (1C5B2) - BSA Free

• ALDA
• aldolase A, fructose-bisphosphate
• EC 4.1.2.13
• fructose-1,6-bisphosphate triosephosphate-lyase
• fructose-bisphosphate aldolase A
• GSD12
• Lung cancer antigen NY-LU-1
• MGC10942
• MGC17716
• MGC17767
• Muscle-type aldolase

Background

The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDOA