Aldolase A Antibodies
We offer Aldolase A Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each Aldolase A Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Aldolase A Antibodies can be used in a variety of model species: Human, Mouse, Rabbit, Rat. Use the list below to choose the Aldolase A Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Aldolase A polyclonal antibodies and browse our Aldolase A monoclonal antibody catalog.
Alternate Names for Aldolase A Antibodies
anti-Aldolase A antibody, anti-ALDOA antibody, anti-ALDA antibody, anti-aldolase A, fructose-bisphosphate antibody, anti-EC 4.1.2.13 antibody, anti-fructose-1,6-bisphosphate triosephosphate-lyase antibody, anti-fructose-bisphosphate aldolase A antibody, anti-GSD12 antibody, anti-Lung cancer antigen NY-LU-1 antibody, anti-MGC10942 antibody, anti-MGC17716 antibody, anti-MGC17767 antibody, anti-Muscle-type aldolase antibody
12 Results for "aldolase-a" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: Aldolase A Antibody [NBP1-87488] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87488-img0008.jpg "Immunocytochemistry/Immunofluorescence: Aldolase A Antibody [NBP1-87488] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.")
![Aldolase A Antibody [NBP1-87488] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/antibody/nbp1-87488_rabbit-polyclonal-aldolase-a-antibody-immunohistochemistry-paraffin-1852023111210..jpg)
![Aldolase A Antibody [NBP1-87488] -Staining of human kidney shows moderate cytoplasmic positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/antibody/nbp1-87488_rabbit-polyclonal-aldolase-a-antibody-immunohistochemistry-paraffin-1852023111334..jpg)
![Aldolase A Antibody [NBP1-87488] -Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87488_rabbit-polyclonal-aldolase-a-antibody-immunohistochemistry-paraffin-1852023111457..jpg)
![Aldolase A Antibody [NBP1-87488] -Staining of human testis shows weak cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87488_rabbit-polyclonal-aldolase-a-antibody-immunohistochemistry-paraffin-1852023111636..jpg)
![Aldolase A Antibody [NBP1-87488] -Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/antibody/nbp1-87488_rabbit-polyclonal-aldolase-a-antibody-western-blot-1852023111745..jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - Analysis using Biotin conjugate of NB600-915. 300 ul aliquots of whole anti-aldolase antiserum used to precipitate varying amounts of purified aldolase & precipitates with controls; compared by SDS-PAGE & Western blot. 1. Purified aldolase 2. 300 ul antiserum with no antigen (negative control) 3. 300 ul antiserum with 100 ul aldolase (2.5 mg/ml) 4. 300 ul antiserum with 200 ul aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum & an equal volume of aldolase antigen in PBS incubated 24 hrs at 4C, centrifuged for 6 mins at 13K RPM, washed once with PBS, centrifuged & dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, sample was divided in 2 portions, & an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer added. The reduced samples were boiled for five mins, & all samples were run at 140 V for 45 mins on a 4-20% tris/glycine gradient gel. Gel was stained, destained & imaged.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0010.jpg "Western Blot: Aldolase A Antibody [NB600-915] - Analysis using Biotin conjugate of NB600-915. 300 ul aliquots of whole anti-aldolase antiserum used to precipitate varying amounts of purified aldolase & precipitates with controls; compared by SDS-PAGE & Western blot. 1. Purified aldolase 2. 300 ul antiserum with no antigen (negative control) 3. 300 ul antiserum with 100 ul aldolase (2.5 mg/ml) 4. 300 ul antiserum with 200 ul aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum & an equal volume of aldolase antigen in PBS incubated 24 hrs at 4C, centrifuged for 6 mins at 13K RPM, washed once with PBS, centrifuged & dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, sample was divided in 2 portions, & an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer added. The reduced samples were boiled for five mins, & all samples were run at 140 V for 45 mins on a 4-20% tris/glycine gradient gel. Gel was stained, destained & imaged.")
![Western Blot: Aldolase A Antibody [NB600-915] - Figure. Immunoblotting. IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4 degrees C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0003.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4 C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE).](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Western-Blot-NB600-915-img0009.jpg)
![Immunoprecipitation: Aldolase A Antibody [NB600-915] - Analysis using the Biotin conjugate of NB600-915. Detection of 300 ul of anti aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 ug/ml) of purified aldolase in PBS. Precipitation of aldolase was confirmed.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-Immunoprecipitation-NB600-915-img0005.jpg)
![Western Blot: Aldolase A Antibody [NB600-915] - Twenty one more markedly oxidized proteins identified in 6‐week‐old PLP‐tg66/66 mice in the spinal cord. All non‐mitochondrial oxidized proteins tested by Western blot in the spinal cord are normally expressed. Relative protein level expressed as a percentage of control and in reference to gamma ‐tubulin as a loading marker (n = 6/genotype). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29027761/) licensed under a CC-BY license](http://images.novusbio.com/fullsize/nb600-915_goat-polyclonal-aldolase-a-antibody-2552023153959.jpg)
![Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mAb against HEK293 (1) and ALDOA (AA: 9-145)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Western-Blot-NBP2-61844-img0006.jpg "Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mAb against HEK293 (1) and ALDOA (AA: 9-145)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis of breast cancer tissues using ALDOA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Immunohistochemistry-NBP2-61844-img0004.jpg)
![Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mAb against human ALDOA (AA: 9-145) recombinant protein. (Expected MW is 40.7 kDa)](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Western-Blot-NBP2-61844-img0005.jpg)
![Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mouse mAb against MCF-7 (1), Hela (2), and NIH/3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Western-Blot-NBP2-61844-img0007.jpg)
![Immunohistochemistry-Paraffin: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis of bladder cancer tissues using ALDOA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Immunohistochemistry-NBP2-61844-img0003.jpg)
![ELISA: Aldolase A Antibody (1C5B2) [NBP2-61844] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-ELISA-NBP2-61844-img0001.jpg)
![ELISA: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis of K562 cells using ALDOA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-ELISA-NBP2-61844-img0002.jpg)
![Western Blot: Aldolase A Antibody (4B2E5) [NBP3-15381] - Western blot analysis of extracts of various cell lines, using Aldolase A Rabbit mAb (NBP3-15381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-4B2E5-Western-Blot-NBP3-15381-img0003.jpg "Western Blot: Aldolase A Antibody (4B2E5) [NBP3-15381] - Western blot analysis of extracts of various cell lines, using Aldolase A Rabbit mAb (NBP3-15381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Aldolase A Antibody (4B2E5) [NBP3-15381] -Analysis of paraffin-embedded Human tonsil tissue using ALDOA Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15381_rabbit-aldolase-a-mab-4b2e5-immunohistochemistry-paraffin-2510202412298..png)
![Immunohistochemistry-Paraffin: Aldolase A Antibody (4B2E5) [NBP3-15381] -Analysis of paraffin-embedded Mouse heart tissue using ALDOA Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15381_rabbit-aldolase-a-mab-4b2e5-immunohistochemistry-paraffin-25102024123025..png)
![Immunohistochemistry-Paraffin: Aldolase A Antibody (4B2E5) [NBP3-15381] -Analysis of paraffin-embedded Rat colon tissue using ALDOA Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer( pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15381_rabbit-aldolase-a-mab-4b2e5-immunohistochemistry-paraffin-25102024123136..png)
![Immunohistochemistry: Aldolase A Antibody (4B2E5) [Aldolase A] - Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using Aldolase A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15381_rabbit-aldolase-a-mab-4b2e5-52202517235716.jpg)
![Immunohistochemistry: Aldolase A Antibody (4B2E5) [Aldolase A] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Aldolase A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15381_rabbit-aldolase-a-mab-4b2e5-5220251726069.jpg)
![Western Blot: Aldolase A Antibody (3D9-6F3) [H00000226-M01] - Analysis of ALDOA expression in transfected 293T cell line by ALDOA monoclonal antibody (M01), clone 3D9-6F3.Lane 1: ALDOA transfected lysate (Predicted MW: 39.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-3D9-6F3-Western-Blot-H00000226-M01-img0008.jpg "Western Blot: Aldolase A Antibody (3D9-6F3) [H00000226-M01] - Analysis of ALDOA expression in transfected 293T cell line by ALDOA monoclonal antibody (M01), clone 3D9-6F3.Lane 1: ALDOA transfected lysate (Predicted MW: 39.4 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Aldolase A Antibody (3D9-6F3) [H00000226-M01] - Analysis of monoclonal antibody to ALDOA on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-3D9-6F3-Immunocytochemistry-Immunofluorescence-H00000226-M01-img0005.jpg)
![Western Blot: Aldolase A Antibody (3D9-6F3) [H00000226-M01] - ALDOA monoclonal antibody (M01), clone 3D9-6F3 Analysis of ALDOA expression in IMR-32.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-3D9-6F3-Western-Blot-H00000226-M01-img0006.jpg)
![Western Blot: Aldolase A Antibody (3D9-6F3) [H00000226-M01] - ALDOA monoclonal antibody (M01), clone 3D9-6F3. Analysis of ALDOA expression in human omentum, serous carcinoma.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-3D9-6F3-Western-Blot-H00000226-M01-img0007.jpg)
![Western Blot: Aldolase A Antibody (3F9) [NBP2-42620] - The Recombinant Human ALDOA and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human ALDOA Lane 2.: HeLa cell lysate Lane 3.: HepG2 cell lysate](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-3F9-Western-Blot-NBP2-42620-img0001.jpg "Western Blot: Aldolase A Antibody (3F9) [NBP2-42620] - The Recombinant Human ALDOA and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human ALDOA Lane 2.: HeLa cell lysate Lane 3.: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: Aldolase A Antibody (3F9) [NBP2-42620] - Analysis of ALDOA in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human A549 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-3F9-Immunocytochemistry-Immunofluorescence-NBP2-42620-img0002.jpg)
![Flow Cytometry: Aldolase A Antibody (3F9) [NBP2-42620] - An intracellular stain was performed on HeLa cells with Aldolase A Antibody (3F9)NBP2-42620AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-3F9-Flow-Cytometry-NBP2-42620-img0004.jpg)
![Flow Cytometry: Aldolase A Antibody (3F9) [NBP2-42620] - Analysis of ALDOA in A549 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-3F9-Flow-Cytometry-NBP2-42620-img0003.jpg)
![Immunohistochemistry: Aldolase A Antibody (7D8) [NBP3-26511] - Image of Aldolase A Antibody (7D8) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26511_rabbit-aldolase-a-mab-7d8-262202415355719.jpg "Immunohistochemistry: Aldolase A Antibody (7D8) [NBP3-26511] - Image of Aldolase A Antibody (7D8) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Aldolase A Antibody (7D8) [NBP3-26511] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, Mouse Liver whole cell lysate, Rat Liver whole cell lysate. All lanes: Aldolase A Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40, 46 kDa Observed band size: 40 kDa](http://images.novusbio.com/fullsize/nbp3-26511_rabbit-aldolase-a-mab-7d8-282202411472126.jpg)
![Immunocytochemistry/Immunofluorescence: Aldolase A Antibody (7D8) [NBP3-26511] - Staining of Hela Cells with Aldolase A Antibody (7D8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26511_rabbit-aldolase-a-mab-7d8-28220241323617.jpg)
![Western Blot: Aldolase A Antibody (3C12-6D11) [H00000226-M02] - Analysis of ALDOA expression in transfected 293T cell line by ALDOA monoclonal antibody (M02), clone 3C12-6D11.Lane 1: ALDOA transfected lysate(39 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-3C12-6D11-Western-Blot-H00000226-M02-img0007.jpg "Western Blot: Aldolase A Antibody (3C12-6D11) [H00000226-M02] - Analysis of ALDOA expression in transfected 293T cell line by ALDOA monoclonal antibody (M02), clone 3C12-6D11.Lane 1: ALDOA transfected lysate(39 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Aldolase A Antibody (3C12-6D11) [H00000226-M02] - Analysis of monoclonal antibody to ALDOA on A-431 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-3C12-6D11-Immunocytochemistry-Immunofluorescence-H00000226-M02-img0005.jpg)
![Western Blot: Aldolase A Antibody (3C12-6D11) [H00000226-M02] - ALDOA monoclonal antibody (M01), clone 3C12-6D11 Analysis of ALDOA expression in A-431.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-3C12-6D11-Western-Blot-H00000226-M02-img0006.jpg)
![Western Blot: Aldolase A Antibody (2E6) [H00000226-M03] - Analysis of ALDOA expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-2E6-Western-Blot-H00000226-M03-img0003.jpg "Western Blot: Aldolase A Antibody (2E6) [H00000226-M03] - Analysis of ALDOA expression in NIH/3T3 (Cat # L018V1).")
![Western Blot: Aldolase A Antibody (2E6) [H00000226-M03] - Analysis of ALDOA expression in HepG2 (Cat # L019V1).](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-2E6-Western-Blot-H00000226-M03-img0002.jpg)
![Western Blot:[NBP3-29624]-Detection of human and mouse by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29624_rabbit-aldolase-a-pab-231202511585561.jpg "Western Blot:[NBP3-29624]-Detection of human and mouse by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation:Aldolase A AntibodyNBP3-30648] -Detection of human ALDOA by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ALDOA antibody NBP3-30648 (lot NBP3-30648-1) used for IP at 6 µg per reaction. ALDOA was also immunoprecipitated by rabbit anti-ALDOA antibody. For blotting immunoprecipitated ALDOA, NBP3-30648 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30648_rabbit-aldolase-a-pab-301220241382411.jpg "Immunoprecipitation:Aldolase A AntibodyNBP3-30648] -Detection of human ALDOA by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ALDOA antibody NBP3-30648 (lot NBP3-30648-1) used for IP at 6 µg per reaction. ALDOA was also immunoprecipitated by rabbit anti-ALDOA antibody. For blotting immunoprecipitated ALDOA, NBP3-30648 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")