Myosin Light Chain 2 Antibody (3B2)

Images

 
Western Blot: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL2 antibody (1:1000). Proteins were ...read more
Immunocytochemistry/ Immunofluorescence: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Analysis of MYL2 in A431 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI ...read more
Flow Cytometry: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Analysis of MYL2 in A431 cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, XpSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
3B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Myosin Light Chain 2 Antibody (3B2) Summary

Immunogen
Recombinant human MYL2 (1-166aa) purified from E. coli (NP_000423).
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYL2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1x10^6cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
Application Notes
Use in IHC and ICC/IF was reported in a scientific publication (PMID: 23727888).
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP1-30249 in the following applications:

Publications
Read Publications using
NBP1-30249 in the following applications:

  • 1 publication
  • 1 publication
  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Xenopus reactivity reported in scientific literature (PMID: 23727888). Mouse reactivity reported in scientific literature (PMID: 28430962).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Myosin Light Chain 2 Antibody (3B2)

  • cardiac ventricular myosin light chain 2
  • CMH10myosin light chain 2
  • DKFZp779C0562
  • MLC2
  • MLC-2
  • MLC-2v
  • myosin regulatory light chain 2, ventricular/cardiac muscle isoform
  • myosin, light chain 2, regulatory, cardiac, slow
  • myosin, light polypeptide 2, regulatory, cardiac, slow
  • regulatory light chain of myosin
  • RLC of myosin
  • slow cardiac myosin regulatory light chain 2

Background

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. Human myosin light chain has clinical application as a cardiac marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myosin Light Chain 2 Antibody (NBP1-30249)(4)

Review for Myosin Light Chain 2 Antibody (NBP1-30249) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-30249:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Myosin Light Chain 2 NBP1-30249
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reviewed by:
Maria Rubio
WB Human 05/26/2011
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Summary

ApplicationWestern Blot
Sample TestedHuman brain homogenate, Sample Amount: 20ug
SpeciesHuman
CommentsI have a specific band at 20kD and many non-specific bands. No bands were observed when blocked with li-cor buffer.

Blocking

Blocking DetailsBlocking Buffer: 5% BSA in PBS, Blocking Time: 1 hour, Blocking Temp: 23 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Incubation Dilution Buffer: BSA1% in PBS, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: infrared, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: Odyssey, Specific Bands: 20 kDa, Non-Specific Bands: 15,35,40,50 kDa

Comments

CommentsI have a specific band at 20kD and many non-specific bands. No bands were observed when blocked with li-cor buffer.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Myosin Light Chain 2 Antibody (NBP1-30249) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-30249AF350
Alexa Fluor 405 NBP1-30249AF405
Alexa Fluor 488 NBP1-30249AF488
Alexa Fluor 532 NBP1-30249AF532
Alexa Fluor 594 NBP1-30249AF594
Alexa Fluor 647 NBP1-30249AF647
Alexa Fluor 700 NBP1-30249AF700
Alexa Fluor 750 NBP1-30249AF750
Allophycocyanin NBP1-30249APC
Allophycocyanin/Cy7 NBP1-30249APCCY7
Biotin NBP1-30249B
DyLight 350 NBP1-30249UV
DyLight 405 NBP1-30249V
DyLight 488 NBP1-30249G
DyLight 550 NBP1-30249R
DyLight 594 NBP1-30249DL594
DyLight 650 NBP1-30249C
DyLight 680 NBP1-30249FR
DyLight 755 NBP1-30249IR
FITC NBP1-30249F
HRP NBP1-30249H
Janelia Fluor 549 NBP1-30249JF549
Janelia Fluor 646 NBP1-30249JF646
PE NBP1-30249PE
PE/Atto594 NBP1-30249PEATT594
PE/Cy5.5 NBP1-30249PECY55
PE/Cy7 NBP1-30249PECY7
PerCP NBP1-30249PCP

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Recent Reviews

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Maria Rubio
05/26/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol MYL2
Entrez
Uniprot