Myosin Light Chain 2 Antibody (3B2)

Images

 
Western Blot: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - The extracts of Rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized ...read more
Immunocytochemistry/ Immunofluorescence: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Analysis in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MYL2 antibody (1:100) with ...read more
Flow Cytometry: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Flow cytometry analysis of MYL2 in A431 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, XpSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
3B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Myosin Light Chain 2 Antibody (3B2) Summary

Immunogen
Recombinant human MYL2 (1-166aa) purified from E. coli (NP_000423).
Specificity
This antibody is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MYL2 protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYL2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
Application Notes
Use in Immunohistochemistry and Immunocytochemistry/Immunofluorescence was reported in a scientific publication (PMID: 23727888).
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP1-30249 in the following applications:

Publications
Read Publications using
NBP1-30249 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Xenopus reactivity reported in scientific literature (PMID: 23727888). Mouse reactivity reported in scientific literature (PMID: 28430962).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Myosin Light Chain 2 Antibody (3B2)

  • cardiac ventricular myosin light chain 2
  • CMH10myosin light chain 2
  • DKFZp779C0562
  • MLC2
  • MLC-2
  • MLC-2v
  • myosin regulatory light chain 2, ventricular/cardiac muscle isoform
  • myosin, light chain 2, regulatory, cardiac, slow
  • myosin, light polypeptide 2, regulatory, cardiac, slow
  • regulatory light chain of myosin
  • RLC of myosin
  • slow cardiac myosin regulatory light chain 2

Background

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. Human myosin light chain has clinical application as a cardiac marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myosin Light Chain 2 Antibody (NBP1-30249)(3)

Review for Myosin Light Chain 2 Antibody (NBP1-30249) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-30249:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Myosin Light Chain 2 NBP1-30249
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reviewed by:
Maria Rubio
WB Human 05/26/2011
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Summary

ApplicationWestern Blot
Sample TestedHuman brain homogenate, Sample Amount: 20ug
SpeciesHuman
CommentsI have a specific band at 20kD and many non-specific bands. No bands were observed when blocked with li-cor buffer.

Blocking

Blocking DetailsBlocking Buffer: 5% BSA in PBS, Blocking Time: 1 hour, Blocking Temp: 23 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Incubation Dilution Buffer: BSA1% in PBS, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: infrared, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: Odyssey, Specific Bands: 20 kDa, Non-Specific Bands: 15,35,40,50 kDa

Comments

CommentsI have a specific band at 20kD and many non-specific bands. No bands were observed when blocked with li-cor buffer.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Myosin Light Chain 2 Antibody (NBP1-30249) (0)

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Secondary Antibodies

 

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Recent Reviews

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Maria Rubio
05/26/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol MYL2
Entrez
Uniprot