Myosin Light Chain 2 Antibody (3B2)

Western Blot: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - The extracts of Rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Analysis in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MYL2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow Cytometry: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Flow cytometry analysis of MYL2 in A431 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

• Reactivity: Hu, Mu, Rt, Xp
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 3B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Myosin Light Chain 2 Antibody (3B2) Summary

• Immunogen: Recombinant human MYL2 (1-166aa) purified from E. coli (NP_000423).
• Specificity: This antibody is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MYL2 protein.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYL2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
• Application Notes: Use in Immunohistochemistry and Immunocytochemistry/Immunofluorescence was reported in a scientific publication (PMID: 23727888).
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus reactivity reported in scientific literature (PMID: 23727888). Mouse reactivity reported in scientific literature (PMID: 28430962).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Myosin Light Chain 2 Antibody (3B2)

• cardiac ventricular myosin light chain 2
• CMH10myosin light chain 2
• DKFZp779C0562
• MLC2
• MLC-2
• MLC-2v
• myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• myosin, light chain 2, regulatory, cardiac, slow
• myosin, light polypeptide 2, regulatory, cardiac, slow
• regulatory light chain of myosin
• RLC of myosin
• slow cardiac myosin regulatory light chain 2

Background

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. Human myosin light chain has clinical application as a cardiac marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myosin Light Chain 2 Antibody (NBP1-30249)(3)

Publications using NBP1-30249

• An M, Ryu DR, Won Park J et al. ULK1 prevents cardiac dysfunction in obesity through autophagy-meditated regulation of lipid metabolism. Cardiovasc. Res. Apr 17 2017 [PMID: 28430962] (WB, Mouse)
• Guo R, Liu G, Du M et al. Early ketamine exposure results in cardiac enlargement and heart dysfunction in Xenopus embryos. BMC Anesthesiol. Apr 19 2016 [PMID: 27091482] (WB, Xenopus)
• Samwer M, Dehne HJ, Spira F et al. The nuclear F-actin interactome of Xenopus oocytes reveals an actin-bundling kinesin that is essential for meiotic cytokinesis. EMBO J 2013 May 31 [PMID: 23727888] (ICC/IF, IHC, Xenopus)

Review for Myosin Light Chain 2 Antibody (NBP1-30249) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Maria Rubio WB Human 05/26/2011

Summary

• Application: Western Blot
• Sample Tested: Human brain homogenate, Sample Amount: 20ug
• Species: Human
• Comments: I have a specific band at 20kD and many non-specific bands. No bands were observed when blocked with li-cor buffer.

Blocking

• Blocking Details: Blocking Buffer: 5% BSA in PBS, Blocking Time: 1 hour, Blocking Temp: 23 degrees Celsius

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:500, Incubation Dilution Buffer: BSA1% in PBS, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

• Secondary Description: Secondary Ab: infrared, Secondary Ab Dilution Ratio: 1:5000

Details

• Detection Notes: Detection Method: Odyssey, Specific Bands: 20 kDa, Non-Specific Bands: 15,35,40,50 kDa

Comments

• Comments: I have a specific band at 20kD and many non-specific bands. No bands were observed when blocked with li-cor buffer.

Bioinformatics

• Gene Symbol: MYL2
• Entrez:
  • 4633(Human)
  • 363925(Rat)
• Uniprot:
  • P10916(Human)