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Myosin Light Chain 2 Antibody (3B2)

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Western Blot: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - The extracts of Rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - The extracts of Rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized ...read more
Immunocytochemistry/ Immunofluorescence: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Analysis in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MYL2 antibody (1:100) with ...read more
Flow Cytometry: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Flow cytometry analysis of MYL2 in A431 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, XpSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
3B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-30249AF405
Alexa Fluor 488
NBP1-30249AF488
Alexa Fluor 647
NBP1-30249AF647
Alexa Fluor 700
NBP1-30249AF700
Allophycocyanin
NBP1-30249APC
Allophycocyanin/Cy7
NBP1-30249APCCY7
Biotin
NBP1-30249B
DyLight 350
NBP1-30249UV
DyLight 405
NBP1-30249V
DyLight 488
NBP1-30249G
DyLight 550
NBP1-30249R
DyLight 650
NBP1-30249C
DyLight 680
NBP1-30249FR
DyLight 755
NBP1-30249IR
FITC
NBP1-30249F
HRP
NBP1-30249H
Janelia Fluor 549
NBP1-30249JF549
Janelia Fluor 646
NBP1-30249JF646
PE
NBP1-30249PE
PE/Atto594
NBP1-30249PEATT594
PE/Cy5.5
NBP1-30249PECY55
PE/Cy7
NBP1-30249PECY7
PerCP
NBP1-30249PCP
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Myosin Light Chain 2 Antibody (3B2) Summary

Immunogen
Recombinant human MYL2 (1-166aa) purified from E. coli (NP_000423).
Specificity
This antibody is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MYL2 protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYL2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
Application Notes
Use in Immunohistochemistry and Immunocytochemistry/Immunofluorescence was reported in a scientific publication (PMID: 23727888).
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP1-30249 in the following applications:
Publications
Read Publications using
NBP1-30249 in the following applications:

  • ICC/IF
    1 publication
  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes

Xenopus reactivity reported in scientific literature (PMID: 23727888). Mouse reactivity reported in scientific literature (PMID: 28430962).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Myosin Light Chain 2 Antibody (3B2)

  • cardiac ventricular myosin light chain 2
  • CMH10myosin light chain 2
  • DKFZp779C0562
  • MLC2
  • MLC-2
  • MLC-2v
  • myosin regulatory light chain 2, ventricular/cardiac muscle isoform
  • myosin, light chain 2, regulatory, cardiac, slow
  • myosin, light polypeptide 2, regulatory, cardiac, slow
  • regulatory light chain of myosin
  • RLC of myosin
  • slow cardiac myosin regulatory light chain 2

Background

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. Human myosin light chain has clinical application as a cardiac marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: MLC1 Antibody [NBP1-81555] - Staining in human cerebral cortex and pancreas tissues using anti-MLC1 antibody. Corresponding MLC1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MLC1 Antibody [NBP1-81555] - Staining of human cerebral cortex shows high expression.
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Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Myosin Heavy Chain was detected in perfusion fixed frozen sections of mouse skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
(2 Reviews)
(26 Publications)
Species: Multi
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RhoD Antibody
NBP1-58359
Western Blot: RhoD Antibody [NBP1-58359] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry: RhoD Antibody [NBP1-58359] - Human Liver Tissue Observed Staining: Cytoplasm in sinusoids of liver Primary Antibody Concentration: 1 : 100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Expos
Immunohistochemistry-Paraffin: RhoD Antibody [NBP1-58359] - Human Tonsil Tissue, 4-8ug/ml.
Species: Hu
Applications: WB, IHC, IHC-P
Rhodopsin Antibody
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Western Blot: Rhodopsin Antibody (A531) [NBP2-25159] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hydrophobic nature of Rhodopsin, it is important not to boil a sample containing it in SDS-PAGE sample buffer, as this will result in more extensive aggregation of the Rhodopsin protein.
Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (A531) [NBP2-25159] - Pig retinal section stained with NBP2-25159 (green) and counterstained with rabbit polyclonal antibody to NF-M NB300-133 (red) and DNA (blue). Rhodopsin is most abundant in the outer segments of retina (OS), NF-M is abundant in the optic nerve fiber layer (ONFL), but seen in processes and neurons in other regions also. Other layers are pigmented epithelium (PE), outer and inner nuclear layers (ONL, INL), outer and inner plexiform layers (OPL, IPL) and ganglion cell layer (GCL).
(1 Publication)
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Applications: WB, ICC/IF, IHC
Myosin light chain
kinase Antibody (SU40 ...
NBP2-67486
Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of MYLK on human lung lysates using anti-MYLK antibody at 1/5,000 dilution.
Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of paraffin-embedded rat smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Atrial Natriuretic
Peptide/ANP Antibody
NBP2-14873
Western Blot: Atrial Natriuretic Peptide/ANP Antibody [NBP2-14873] - Mouse tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with ANP antibody diluted by 1:5000.
Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody [NBP2-14873] - Paraffin-embedded mouse muscle. ANP antibody [N1C3] ) diluted at 1:500.
Western Blot: Atrial Natriuretic Peptide/ANP Antibody [NBP2-14873] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [N1C3] diluted at 1:2000.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
MYLK3 Antibody
NBP2-62682
Immunohistochemistry-Paraffin: MYLK3 Antibody [NBP2-62682] - Staining of human heart muscle shows high expression.
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Immunohistochemistry-Paraffin: MYLK3 Antibody [NBP2-62682] - Staining of human pancreas shows low expression as expected.
Species: Hu
Applications: IHC, IHC-P
NKX2.5 Antibody
NBP1-31558
Western Blot: NKX2.5 Antibody [NBP1-31558] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: NKX2.5 Antibody [NBP1-31558] - Paraformaldehyde-fixed mouse ESC D3, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP1-31558] - Paraffin-embedded rat heart. Nkx2.5 stained by Nkx2.5 antibody diluted at 1:500.
(2 Publications)
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Applications: WB, Flow, ICC/IF, IHC, IHC-P
MYL7 Antibody
(OTI4E7)
NBP2-03891
Western Blot: MYL7 Antibody (4E7) [NBP2-03891] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYL7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYL7.
Immunohistochemistry-Paraffin: MYL7 Antibody (4E7) [NBP2-03891] Staining of paraffin-embedded Human liver tissue using anti-MYL7 mouse monoclonal antibody.
Flow Cytometry: MYL7 Antibody (4E7) [NBP2-03891] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MYL7 antibody, and then analyzed by flow cytometry.
Species: Hu
Applications: WB, Flow, IHC, IHC-P
Troponin I Type 3
(cardiac) Antibody (1 ...
NB110-2546
Flow Cytometry: Troponin I Type 3 (cardiac) Antibody (19C7) [NB110-2546] - FACS analysis of Troponin I Type 3 in differentiated ESC cultures using anti-Troponin I Type 3 (cardiac) antibody. Image from verified customer review.
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Applications: WB, ChIP, Flow, IA, ICC/IF, IHC, IHC-P, IP, S-ELISA
MYH7 Antibody
(940906) [Unconjugate
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Applications: WB, IHC
MHC Class I
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Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-MHC Class I (OX18) NB120-6405 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
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Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, CyTOF-ready, Flow-IC
Myosin light chain
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Immunohistochemistry-Paraffin: Myosin light chain 3 Antibody [NBP1-88068] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: Myosin light chain 3 Antibody [NBP1-88068] - Analysis in human heart muscle and lymph node tissues. Corresponding MYL3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Myosin light chain 3 Antibody [NBP1-88068] - Staining of human heart muscle shows moderate to strong cytoplasmic positivity in cardiomyocytes.
Species: Hu
Applications: IHC, IHC-P
MyoD Antibody
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Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Analysis for MyoD expression in various small round cell tumor lines using 1 ug/ml anti-MyoD mAb. The antibody only reacts with a band of approx. 45 kD in the rhabdomyosarcoma cell line (Rh30, lane 1) but was negative against the primitive neuroectodermal (PFSK-1A, lane 2), lymphoma (EB2, lane 3), neuroblastoma (SK-N-SH, lane 4), and Ewing's sarcoma (SJSA-1, lane 5) cell lines.
Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - Cell Lines Tested: mouse skeletal muscle-derived primary cell population Test Sample Preparation: mouse skeletal muscle digested by collagenase type 2 System: Super Sensitive High Resolution Confocal Laser Microscope (LSM880 with Airyscan) Excitation Wavelength: 488nm Emission Filter: 562nm Image is courtesy of customer review. Image using the DyLight 488 form of this antibody
Immunohistochemistry-Frozen: MyoD Antibody (5.8A) [NB100-56511] - Clone 5.8A antibody in human tissues. A. Rhabdomyosaroma (nuclei are stained), B. Lymphoma (staining is absent)
(1 Review)
(10 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
MRLC2 Antibody
NBP2-54719
Western Blot: MRCL3 Antibody [NBP2-54719] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/Immunofluorescence: MRLC2 Antibody [NBP2-54719] - MRCL3 Antibody [NBP2-54719] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli fibrillar center.
Immunohistochemistry-Paraffin: MRCL3 Antibody [NBP2-54719] - Staining of human smooth muscle shows moderate cytoplasmic positivity in smooth muscle cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
HESX1 Antibody
NBP1-89090
Immunohistochemistry-Paraffin: HESX1 Antibody [NBP1-89090] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts as well as in Leydig cells.
Immunohistochemistry-Paraffin: HESX1 Antibody [NBP1-89090] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: HESX1 Antibody [NBP1-89090] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.
Species: Hu
Applications: IHC, IHC-P
Troponin C
(cardiac) Antibody
NBP2-55151
Immunocytochemistry/Immunofluorescence: Troponin C (cardiac) Antibody [NBP2-55151] - Staining of human cell line HeLa shows localization to nucleoplasm, mitochondria & actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody [NBP2-55151] - Staining of human liver using Anti-TNNC1 antibody.
Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody [NBP2-55151] - Staining of human heart muscle shows high expression.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Calmodulin 3
Antibody
NBP1-98474
Western Blot: Calmodulin 3 Antibody [NBP1-98474] - Human Jurkat, Antibody Dilution: 1.0 ug/ml CALM3 is strongly supported by BioGPS gene expression data to be expressed in Jurkat.
Western Blot: Calmodulin 3 Antibody [NBP1-98474] - Titration: 1.0 ug/ml Positive Control: Hela Whole Cell.
Western Blot: Calmodulin 3 Antibody [NBP1-98474] - Human 721_B, Antibody Dilution: 1.0 ug/ml CALM3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.
Species: Hu
Applications: WB

Publications for Myosin Light Chain 2 Antibody (NBP1-30249)(3)

We have publications tested in 2 confirmed species: Mouse, Xenopus.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


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ICC/IF
(1)
IHC
(1)
WB
(2)
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Mouse
(1)
Xenopus
(2)
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Review for Myosin Light Chain 2 Antibody (NBP1-30249) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-30249:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot Myosin Light Chain 2 NBP1-30249
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reviewed by:
Maria Rubio
 WB Human 05/26/2011
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Summary

ApplicationWestern Blot
Sample TestedHuman brain homogenate, Sample Amount: 20ug
SpeciesHuman
CommentsI have a specific band at 20kD and many non-specific bands. No bands were observed when blocked with li-cor buffer.

Blocking

Blocking DetailsBlocking Buffer: 5% BSA in PBS, Blocking Time: 1 hour, Blocking Temp: 23 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Incubation Dilution Buffer: BSA1% in PBS, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: infrared, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: Odyssey, Specific Bands: 20 kDa, Non-Specific Bands: 15,35,40,50 kDa

Comments

CommentsI have a specific band at 20kD and many non-specific bands. No bands were observed when blocked with li-cor buffer.

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Western Blot Myosin Light Chain 2 NBP1-30249
Enlarge
 Maria Rubio
05/26/2011
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol MYL2
Entrez
Uniprot