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ALCAM/CD166 Antibody (5B3)

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Western Blot: ALCAM/CD166 Antibody (5B3) [NBP2-22211] - Western blot analysis using CD166 mouse mAb against L1210 (1) cell lysate, and Mouse spleen (2) tissue lysate.
Western Blot: ALCAM/CD166 Antibody (5B3) [NBP2-22211] - Western blot analysis using CD166 mouse mAb against L1210 (1) cell lysate, and Mouse spleen (2) tissue lysate.
Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (5B3) [NBP2-22211] - Immunohistochemical analysis of paraffin-embedded striated muscle tissues using CD166 mouse mAb with DAB staining.
Western Blot: ALCAM/CD166 Antibody (5B3) [NBP2-22211] - Western blot analysis using CD166 mAb against human CD166 recombinant protein. (Expected MW is 26.3 kDa)
ELISA: ALCAM/CD166 Antibody (5B3) [NBP2-22211] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
5B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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ALCAM/CD166 Antibody (5B3) Summary

Immunogen
Purified recombinant fragment of human AlCAM (AA: 405-524) expressed in E. Coli.
Localization
Cytoplasm
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ALCAM
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
65.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for ALCAM/CD166 Antibody (5B3)

  • activated leucocyte cell adhesion molecule
  • activated leukocyte cell adhesion moleculeFLJ38514
  • ALCAM
  • CD166 antigen
  • CD166
  • MEMDMGC71733

Background

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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