ALCAM/CD166 Antibody (5B3) - BSA Free

Western Blot: ALCAM/CD166 Antibody (5B3) [NBP2-22211] - Western blot analysis using CD166 mouse mAb against L1210 (1) cell lysate, and Mouse spleen (2) tissue lysate.

Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (5B3) [NBP2-22211] - Immunohistochemical analysis of paraffin-embedded striated muscle tissues using CD166 mouse mAb with DAB staining.

Western Blot: ALCAM/CD166 Antibody (5B3) [NBP2-22211] - Western blot analysis using CD166 mAb against human CD166 recombinant protein. (Expected MW is 26.3 kDa)

ELISA: ALCAM/CD166 Antibody (5B3) [NBP2-22211] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 5B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ALCAM/CD166 Antibody (5B3) - BSA Free Summary

• Description: Novus Biologicals Mouse ALCAM/CD166 Antibody (5B3) - BSA Free (NBP2-22211) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human ALCAM/CD166 (AA: 405-524) expressed in E. Coli.
• Localization: Cytoplasm
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALCAM
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 65.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for ALCAM/CD166 Antibody (5B3) - BSA Free

• activated leucocyte cell adhesion molecule
• activated leukocyte cell adhesion moleculeFLJ38514
• ALCAM
• CD166 antigen
• CD166
• MEMDMGC71733

Background

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALCAM