ALCAM/CD166 Antibody (21B9) - BSA Free

Immunohistochemistry: ALCAM/CD166 Antibody (21B9) [NBP3-26512] - Image of ALCAM/CD166 Antibody (21B9) diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunohistochemistry: ALCAM/CD166 Antibody (21B9) [NBP3-26512] - Image of ALCAM/CD166 Antibody (21B9) diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot: ALCAM/CD166 Antibody (21B9) [NBP3-26512] - Positive Western Blot detected in: PC3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate, Mouse brain tissue. All lanes: ALCAM/CD166 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 66, 64, 16, 34 kDa Observed band size: 100 kDa

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clone: 21B9
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ALCAM/CD166 Antibody (21B9) - BSA Free Summary

• Description: Novus Biologicals Rabbit ALCAM/CD166 Antibody (21B9) - BSA Free (NBP3-26512) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human ALCAM/CD166 [UniProt Q13740]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ALCAM
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ALCAM/CD166 Antibody (21B9) - BSA Free

• activated leucocyte cell adhesion molecule
• activated leukocyte cell adhesion moleculeFLJ38514
• ALCAM
• CD166 antigen
• CD166
• MEMDMGC71733

Background

Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic andhomophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as ininteractions between cells of the nervous system

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALCAM