AIF-1/Iba1 Antibody (603102) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: IHC
• Clone: 603102
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

AIF-1/Iba1 Antibody (603102) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human AIF-1/Iba1
  Met1-Pro147
  Accession # P55008
• Specificity: Detects human AIF-1/Iba1 in direct ELISAs.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AIF-1/Iba1 Antibody (603102) [Alexa Fluor® 594]

• AIF1
• AIF-1
• allograft inflammatory factor 1
• G1
• IBA1
• IbaI
• interferon gamma responsive transcript
• Ionized calcium-binding adapter molecule 1
• IRT1
• IRT-1
• Protein G1

Background

Allograft-inflammatory factor 1 (AIF-1), also known as daintain and Iba1, is a 17 kDa cytoplasmic protein that contains two calcium-binding EF hand motifs (aa 45-80 and aa 81-115). AIF-1 is expressed in activated macrophages and is important for the proinflammatory response to ischemia, tissue injury, and atherosclerosis. It is also expressed in vascular smooth muscle and endothelial cells in these areas where it promotes vascular remodeling and new vessel formation. AIF-1 is upregulated in allografted tissue during transplant rejection. It also promotes the proliferation of breast cancer cells and in vivo tumorigenesis. The 147 amino acid (aa) isoform 3 of human AIF-1 shares 89% aa sequence identity with mouse and rat AIF-1. Isoform 1 of human AIF-1 lacks the N-terminal 54 aa of isoform 3. Isoform 2 lacks
aa 1-54 as well as aa 121-147 but also has a 66 aa insertion at position 65.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.