Ago2/eIF2C2 Antibody (7C2) - BSA Free

Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (7C2) [NBP3-26520] - Staining of MCF-7 with Ago2/eIF2C2 Antibody (7C2) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 492-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: Ago2/eIF2C2 Antibody (7C2) [NBP3-26520] - Overlay Peak curve showing Hela cells stained with Ago2/eIF2C2 Antibody (7C2) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF
• Clone: 7C2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Ago2/eIF2C2 Antibody (7C2) - BSA Free Summary

• Description: Novus Biologicals Rabbit Ago2/eIF2C2 Antibody (7C2) - BSA Free (NBP3-26520) is a recombinant monoclonal antibody validated for use in ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human Ago2/eIF2C2 [UniProt Q9UKV8]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AGO2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Ago2/eIF2C2 Antibody (7C2) - BSA Free

• AGO2argonaute 2
• argonaute2
• EC 3.1.26.n1
• EC 3.1.26.n2
• eIF2C 2
• eIF-2C 2
• Eukaryotic translation initiation factor 2C 2
• eukaryotic translation initiation factor 2C, 2
• hAgo2
• MGC3183
• PAZ Piwi domain protein
• PPD
• protein argonaute-2
• Protein slicer
• Q10

Background

Ago2 / eIF2C2 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AGO2