S Opsin Antibody - Azide and BSA Free

Images

 
Western Blot: S Opsin Antibody [NBP1-20194] - WB on tissue lysates. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:500 dilution, incubated overnight at 4C.
Immunohistochemistry-Paraffin: S Opsin Antibody [NBP1-20194] - Sections of mouse eye. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. dilution 1: 500, incubated 30 ...read more
Immunohistochemistry-Paraffin: S Opsin Antibody [NBP1-20194] - Sections of mouse eye. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. dilution 1: 500, incubated ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

S Opsin Antibody - Azide and BSA Free Summary

Immunogen
A synthetic peptide from human OPSB conjugated to blue carrier protein was used as the antigen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OPN1SW
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 10-50 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 10-50 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID:32716032).
Reviewed Applications
Read 1 Review rated 5
using
NBP1-20194 in the following applications:

Publications
Read Publications using
NBP1-20194 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Ammonium sulfate precipitation
Reconstitution Instructions
Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Alternate Names for S Opsin Antibody - Azide and BSA Free

  • BCPBlue cone photoreceptor pigmentBlue-sensitive opsin
  • BOPshort-wave-sensitive opsin 1
  • CBT
  • opsin 1 (cone pigments), short-wave-sensitive

Background

FUNCTION: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. May increase spectral sensitivity in dim light.; SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.; TISSUE SPECIFICITY: Expresssed in cone photoreceptor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S Opsin Antibody (NBP1-20194)(6)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: Func, ICC/IF, IHC, IHC-P, WB.


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Func
(1)
ICC/IF
(1)
IHC
(1)
IHC-P
(1)
WB
(1)
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Mouse
(2)
Rat
(1)
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Review for S Opsin Antibody (NBP1-20194) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-20194:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot S Opsin NBP1-20194
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 09/29/2017
View

Summary

ApplicationWestern Blot
Sample Testedmouse retina
SpeciesMouse
LotRb0946-1-240509-WS

Comments

Commentsdilution ratio is 1:200

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for S Opsin Antibody (NBP1-20194). (Showing 1 - 1 of 1 FAQs).

  1. What is the immunogen sequence of NBP1-20194?
    • For NBP1-20194, the lab informed me that the immunogen is located in the amino acid region of 200-300 of human Opsin B, it is homologous in many species including guinea pig.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
09/29/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol OPN1SW
Entrez
Uniprot