S Opsin Antibody - Azide and BSA Free

Western Blot: S Opsin Antibody [NBP1-20194] - WB on tissue lysates. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:500 dilution, incubated overnight at 4C.

Immunohistochemistry-Paraffin: S Opsin Antibody [NBP1-20194] - Sections of mouse eye. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: S Opsin Antibody [NBP1-20194] - Sections of mouse eye. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

S Opsin Antibody - Azide and BSA Free Summary

• Immunogen: A synthetic peptide from human OPSB conjugated to blue carrier protein was used as the antigen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OPN1SW
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 10-50 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 10-50 ug/ml
• Application Notes: Use in ICC/IF reported in scientific literature (PMID:32716032).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Ammonium sulfate precipitation
• Reconstitution Instructions: Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Alternate Names for S Opsin Antibody - Azide and BSA Free

• BCPBlue cone photoreceptor pigmentBlue-sensitive opsin
• BOPshort-wave-sensitive opsin 1
• CBT
• opsin 1 (cone pigments), short-wave-sensitive

Background

FUNCTION: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. May increase spectral sensitivity in dim light.; SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.; TISSUE SPECIFICITY: Expresssed in cone photoreceptor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S Opsin Antibody (NBP1-20194)(3)

Publications using NBP1-20194

• Dezfuly AR, Safaee A, Amirpour N et al. Therapeutic effects of human adipose mesenchymal stem cells and their paracrine agents on sodium iodate induced retinal degeneration in rats Life sciences [PMID: 35469914] (IHC-P, Rat)
• Strayve D, Makia MM, Kakakhel M et al. ROM1 contributes to phenotypic heterogeneity in PRPH2-associated retinal disease Hum. Mol. Genet. 2020-07-27 [PMID: 32716032] (ICC/IF)
• Sharif AS, Yu D, Loertscher S, Austin R. C8ORF37 is required for photoreceptor outer segment disc morphogenesis by maintaining outer segment membrane protein homeostasis. J. Neurosci. 2018-02-13 [PMID: 29440555] (Mouse)

Review for S Opsin Antibody (NBP1-20194) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Jun Yang WB Mouse 09/29/2017

Summary

• Application: Western Blot
• Sample Tested: mouse retina
• Species: Mouse
• Lot: Rb0946-1-240509-WS

Comments

• Comments: dilution ratio is 1:200

FAQs for S Opsin Antibody (NBP1-20194). (Showing 1 - 1 of 1 FAQs).

1. What is the immunogen sequence of NBP1-20194?
   • For NBP1-20194, the lab informed me that the immunogen is located in the amino acid region of 200-300 of human Opsin B, it is homologous in many species including guinea pig.

Bioinformatics

• Gene Symbol: OPN1SW
• Entrez:
  • 611(Human)
  • 12057(Mouse)
  • 81644(Rat)
• Uniprot:
  • P03999(Human)