Ago2 antibodies and dicer-independent biogenesis of miRNA

Fri, 07/16/2010 - 07:58

Ago2, also called eIF2C2, antibody is one of 37 reagents targeted to the Argonaute protein family that we at Novus Biologicals have in our antibody catalogue. Argonaute proteins are encoded by genes which play an important role in regulating the control of gene expression by miRNA (microRNA). Recently, a new antibody study showed that the role of Ago2 may be to help generate production of miRNA, rather than simply moderate its function.

Micro RNAs are short, highly conserved RNA molecules that regulate gene expression by binding to complementary 3’ UTR (three prime untranslated regions) of the relevant messenger RNA (mRNA). They were first described in 1993, but it was not until the 2000s that they were recognized as a distinct bio-regulatory group.

Immunohistochemistry: Ago2 / eIF2C2 Antibody Immunohistochemistry: Ago2 / eIF2C2 Antibody

In recent years, a number of antibodystudies have shown the fundamental role miRNAs have in controlling biological processes via up and down regulation of gene expression. They are widely distributed in cells and tissues, with over 1000 human miRNA molecules each controlling multiple genes. Studies have shown that miRNAs are key regulators in a number of cellular processes, including cell proliferation; apoptosis and early development.

Ago2 is thought to work with the dicer enzyme to shorten miRNA, creating the “mature” form of the molecule. However, the recent study, conducted by Hannon, et al. showed a separate pathway, where Ago2 cleaved and shortened pre-miRNA independently of dicer. The study, performed in embryonic mice, showed Ago2 was essential for embryonic development, and pointed to a conserved mechanism of microRNA biogenesis directly dependent on Ago2 catalysis.

Overexpression of miRNA has been linked to lymphocytic leukaemia and other cancers. They are thought to be as important as transcription factors in disease research, providing great potential for antibody suppliers like us at Novus Biologicals.

Novus Biologicals offers many Ago2 reagents for your research needs including:


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