AGER Antibody (JF0975)

Western Blot: AGER Antibody (JF0975) [NBP2-67095] - Analysis of RAGE on mouse lung lysates using anti-RAGE antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: AGER Antibody (JF0975) [NBP2-67095] - Staining RAGE in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: AGER Antibody (JF0975) [NBP2-67095] - Mouse lung cancer tissue stained with AGER antibody. IHC-P image submitted by a verified customer review.

Flow Cytometry: AGER Antibody (JF0975) [NBP2-67095] - Analysis of Jurkat cells with RAGE antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: AGER Antibody (JF0975) [NBP2-67095] - Staining RAGE in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: AGER Antibody (JF0975) [NBP2-67095] - Analysis of paraffin-embedded mouse lung tissue using anti-RAGE antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: JF0975
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

AGER Antibody (JF0975) Summary

• Description: Novus Biologicals Rabbit AGER Antibody (JF0975) (NBP2-67095) is a recombinant monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human AGER aa 350-390. (SwissProt: Q15109 Human; SwissProt: Q62151 Mouse; SwissProt: Q63495 Rat)
• Localization: Cell membrane, Secreted.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AGER
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for AGER Antibody (JF0975)

• advanced glycosylation end product-specific receptor
• AGER
• RAGE isoform delta
• RAGE isoform sRAGE-delta
• RAGE
• Receptor for advanced glycosylation end products
• receptor for advanced glycosylation end-products
• SCARJ1

Background

AGER encodes a member of the immunoglobulin superfamily of cell surface molecules. It is a receptor for various molecules, including the amyloidogenic form of serum amyloid A, amyloid-beta protein, members of the S100/calgranulin superfamily and advanced glycation end products. The gene lies within the major histocompatibility complex (MHC) class III region on chromosome 6. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for AGER Antibody (NBP2-67095) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 12/07/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Lung cancer tissue
• Species: Mouse
• Lot: JF0975

Bioinformatics

• Gene Symbol: AGER