AGER: Novus Biologicals

DRG00

Human RAGE Quantikine ELISA Kit

(87 Publications)

1 Kit

DRG00

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
HRP	PDRG00
HRP	SRG00

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MAB1179

AGER Antibody (175410) [Unconjugated]

(67 Publications)

100 μg

MAB1179

HAF005). Specific bands were detected for RAGE and mature RAGE at approximately 50 and 38 kDa, respectively (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat RAGE Monoclonal Antibody (Catalog # 1179) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005). Specific bands were detected for RAGE and mature RAGE at approximately 50 and 38 kDa, respectively (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat RAGE Monoclonal Antibody (Catalog # 1179) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for RAGE and mature RAGE at approximately 50 and 38 kDa, respectively (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

RAGE was detected in perfusion fixed frozen sections of adult mouse lung using Rat Anti-Mouse/Rat RAGE Monoclonal Antibody (Catalog # MAB1179) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1179U-100UG
Alexa Fluor 405	FAB1179V-100UG
Alexa Fluor 488	FAB1179G-100UG
Alexa Fluor 532	FAB1179X-100UG
Alexa Fluor 594	FAB1179T-100UG
Alexa Fluor 647	FAB1179R-100UG
Alexa Fluor 700	FAB1179N-100UG
Alexa Fluor 750	FAB1179S-100UG
Alexa Fluor Plus 405	FAB1179AFP405-100UG
Alexa Fluor Plus 488	FAB1179AFP488-100UG
Alexa Fluor Plus 555	FAB1179AFP555-100UG
Alexa Fluor Plus 594	FAB1179AFP594-100UG
Alexa Fluor Plus 647	FAB1179AFP647-100UG
Alexa Fluor Plus 680	FAB1179AFP680-100UG
Allophycocyanin	FAB1179A
Biotin	FAB1179B
CoraFluor 1	FAB1179CL1
DyLight 350	FAB1179D
DyLight 405	FAB1179E
DyLight 488	FAB1179K
DyLight 550	FAB1179L
DyLight 594	FAB1179M
DyLight 650	FAB1179W
DyLight 680	FAB1179Y
DyLight 755	FAB1179Z
FITC	FAB1179F
HRP	FAB1179H
Janelia Fluor 525	FAB1179JF525
Janelia Fluor 549	FAB1179I
Janelia Fluor 646	FAB1179J
Janelia Fluor 669	FAB1179JF669
PE	FAB1179P
PerCP	FAB1179C
mFluor Violet 450 SE	FAB1179MFV450
mFluor Violet 500 SE	FAB1179MFV500
mFluor Violet 610 SE	FAB1179MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1179-SP
Carrier Free	MAB1179-500

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AF1145

AGER Antibody [Unconjugated]

(42 Publications)

100 μg

AF1145

HAF017). Specific bands were detected for RAGE at approximately 45-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human, mouse, and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). Specific bands were detected for RAGE at approximately 45-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human, mouse, and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for RAGE at approximately 45-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted using Immunoblot Buffer Group 5." class="big_lightbox">

Western blot shows lysates of human lung tissue and HEK001 human epidermal keratinocyte cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted using Immunoblot Buffer Group 5." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

RAGE was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cerebellum) using 15 µg/mL Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

RAGE was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

RAGE expression mediates cell aggregation through homophilic interactions.(A) Western blot analysis on control preB 300.19 cells (preB/pCAGS) or expressing FL-RAGE (preB/FL-RAGE) using alpha RAGE N-term1 antibody. Forty µg of cell lysate were loaded and detection of GAPDH was used as loading control. (B) Cell aggregation assay. preB/pCAGS or preB/FL-RAGE cells were cultured for 2, 6 or 24 hours before being photographed at 20× magnification in phase contrast (left panel). Bar corresponds to 50 µm. Quantification of cell aggregates area (right panel). Results are displayed as means±SEM (*, P<0.05; **, P<0.01; ***, P<0.0001). (C) Mixed cell aggregation assay. Red preB (Red-preB) or preB/FL-RAGE (expressing GFP; preB/FL-RAGE-GFP) cells were grown as single cell line suspension or mixed in equal number for 24 hours. Red-preB/FL-RAGE-GFP were mixed with preB/FL-RAGE-GFP for the same time. Bar corresponds to 50 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24475194), licensed under a CC-BY license. Not internally tested by R&D Systems.

RAGE expression enhances cell-matrix adhesion.(A) Western blot analysis on rat lung lysate and R3/1 cells using three different antibodies recognizing RAGE extracellular (anti-RAGE N-term1 and anti-RAGE N-term2) or intracellular (anti-RAGE C-term) domains. Asterisk (*) indicates nonspecific bands. Forty µg of cell lysate were loaded and detection of GAPDH was used as loading control. (B) Western blot analysis on R3/1-pLXSN and R3/1-FL-RAGE cells using anti-RAGE N-term1 antibody. Forty µg of cell lysate were loaded and detection of GAPDH was used as loading control. (C, D) Cell-matrix adhesion assay. Adhesion of R3/1-pLXSN or R3/1-FL-RAGE cells onto culture dishes coated with 10 µg/ml ECM proteins. Adhesion to PBS, collagen I (Coll I), Fibronectin (FN), or Laminin (Lam) was assayed for 15 minutes (C) or 45 minutes (D). One representative experiment out of three is shown. Results from triplicate wells are displayed as means±SEM (ns, not significant; *, P<0.05; **, P<0.01). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24475194), licensed under a CC-BY license. Not internally tested by R&D Systems.

Differential expression of RAGE and S100A8/A9 on protein level in whole skin.The expression level was analysed by immunochistochemistry using specific anti human S100A8/A9 and anti human RAGE antibodies. The expression was tested in 5 patient samples per group (IC and OTR with in situ or invasive SCC). a: Expression of RAGE and S100A8/A9 in IC and OTR patients with in situ SCC in comparison to normal skin. b: Expression of RAGE and S100A8/A9 in IC and OTR patient with invasive SCC in comparison to normal skin. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120971), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Exogenous S100A8/A9 induces keratinocyte proliferation.a: Assessment of the proliferation by BrdU assay. To assess the role of S100A8/A9 in normal primary, AK and SCC cultures, they were treated with purified S100A8/A9 for 24 hours and proliferation was assessed by BrdU incorporation. The induction of cellular proliferation was between 30–70% (2 way Anova, p<0.0001). b: Assessment of the proliferation by the incorporation of BrdU analysed by flow cytrometry. Normal keratinocytes were treated with S100A8/A9 (10ng/ml) and BrdU for 24 hours. Afterwards cells were fixed in 4% PFA, permeabilized with 0.5% Triton and stained for RAGE and BrdU using the antibodies mentioned above. The differential BrdU incorporation was compared to untreated cell. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120971), licensed under a CC-BY license. Not internally tested by R&D Systems.

RAGE expression contributes to cell-cell adhesion.(A) Western blot analysis on control HEK cells (HEK/pcDNA) or expressing FL-RAGE (HEK/FL-RAGE) using anti-RAGE N-term1 antibody. Forty µg of cell lysate were loaded and detection of GAPDH was used as loading control. (B) A dissociation assay was performed by applying mechanical force on monolayers of HEK/pcDNA or HEK/FL-RAGE cells. Photographs were taken at 40× magnification in phase contrast. Bar corresponds to 50 µm. The dissociation index is the means±SEM of three independent experiments (**, P<0.01). (C) Localization of FL-RAGE on HEK/pcDNA, HEK/FL-RAGE or a mix of both cell lines (orange). Nuclei are stained in blue. Red arrows indicate HEK/pcDNA cells in contact with HEK/FL-RAGE. Green arrows indicate HEK/FL-RAGE cells in contact with each other. Bar corresponds to 10 µm. (D) Localization of FL-RAGE on R3/1-pLXSN, R3/1-FL-RAGE or a mix of both cell lines (orange). Nuclei are stained in blue. Red arrows indicate R3/1-pLXSN cells in contact with R3/1-FL-RAGE. Green arrows indicates R3/1-FL-RAGE cells in contact with each other. Red arrows indicate cells not expressing RAGE in contact with cells expressing RAGE. Bar corresponds to 10 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24475194), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>The receptor RAGE critically mediates the cellular response to S100A8/A9.a: Direct blockade by a specific RAGE blocking antibody reduces cellular proliferation. RAGE dependent proliferation was analyzed in normal primary, AK and SCC cells. Cells were incubated for 1 hour with a blocking anti-RAGE antibody (80μg/ml, as recommended by manufacturer) followed by S100A8/A9 stimulation for additional 24 hours. The differences in the proliferation after the blockade were assessed by BrdU incorporation (1 way Anova, Bonferroni`s Multiple test, **p<0.05, ***p<0.05). b: Knockdown of RAGE using shRNA reduces cellular proliferation. The knockdown studies were performed using specific lentiviral shRNA against RAGE. Primary keratinocytes were infected by shRAGE and sh control viral particles. Selection of positive clones was performed by puromycine selection. Cells were grown to optimal confluence and were stimulated with 10ng/ml S100A8/A9.Cells with RAGE knockdown showed a delay in proliferation in comparison to control as assessed by BrdU (70%) (1 way Anova, Bonferroni`s Multiple test p****<0.0001) and microscope images. Exogenous S100A8/A9 did not induce proliferation of shRAGE keratinocytes, but only in the control. c: Blocking TLR4 using specific blocking antibody (HTA125) does not impair cellular proliferation. AK cells were treated with a specific blocking TLR4 antibody (HTA125). AK cells were grown for 24 hours in the presence of HTA125 antibody (1μg/ml) and S100A8/A9 (10ng/ml). For detection of the cellular proliferation rate BrdU proliferation assay was performed. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120971), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemical analysis of murine pancreatic tumors generated from KPC #5508 cells. (A) Staining for HMGB1. (B) Staining for RAGE. (C) Control staining where the primary antibody was omitted. In (A–C), the regions defined in the yellow rectangles are shown at higher magnification. Arrows indicate (A) staining for HMGB1 in the luminal part of the cells and (B) cell surface staining for RAGE. All slides come from the same tumor block. Scale bar: 50 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33915939), licensed under a CC-BY license. Not internally tested by R&D Systems.

Native rat lung tissue stained for RAGE/AGER (red) and Acta2 (green). Image from a verified customer review.

1145-RG) was serially diluted and captured by Mouse Anti-Human RAGE/AGER Monoclonal Antibody (Catalog # MAB11451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse/Rat RAGE/AGER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human RAGE/AGER Fc Chimera (Catalog # <a class=

1145-RG) was serially diluted and captured by Mouse Anti-Human RAGE/AGER Monoclonal Antibody (Catalog # MAB11451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse/Rat RAGE/AGER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1145U-100UG
Alexa Fluor 405	AF1145V-100UG
Alexa Fluor 488	AF1145G-100UG
Alexa Fluor 532	AF1145X-100UG
Alexa Fluor 594	AF1145T-100UG
Alexa Fluor 647	AF1145R-100UG
Alexa Fluor 700	AF1145N-100UG
Alexa Fluor 750	AF1145S-100UG
Alexa Fluor Plus 405	AF1145AFP405-100UG
Alexa Fluor Plus 488	AF1145AFP488-100UG
Alexa Fluor Plus 555	AF1145AFP555-100UG
Alexa Fluor Plus 594	AF1145AFP594-100UG
Alexa Fluor Plus 647	AF1145AFP647-100UG
Alexa Fluor Plus 680	AF1145AFP680-100UG
Allophycocyanin	AF1145A
Biotin	BAF1145
CoraFluor 1	AF1145CL1
DyLight 350	AF1145D
DyLight 405	AF1145E
DyLight 488	AF1145K
DyLight 550	AF1145L
DyLight 594	AF1145M
DyLight 650	AF1145W
DyLight 680	AF1145Y
DyLight 755	AF1145Z
FITC	AF1145F
HRP	AF1145H
Janelia Fluor 525	AF1145JF525
Janelia Fluor 549	AF1145I
Janelia Fluor 646	AF1145J
Janelia Fluor 669	AF1145JF669
PE	AF1145P
PerCP	AF1145C
mFluor Violet 450 SE	AF1145MFV450
mFluor Violet 500 SE	AF1145MFV500
mFluor Violet 610 SE	AF1145MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1145-SP

compare

(select up to 3 products from this page to compare)

AF1179

AGER Antibody [Unconjugated]

(34 Publications)

100 μg

AF1179

HAF017). A specific band was detected for RAGE at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1179) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for RAGE at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1179) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RAGE at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). Specific bands were detected for RAGE at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1179) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). Specific bands were detected for RAGE at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

RAGE was detected in immersion fixed frozen sections of mouse brain using Goat Anti-Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1179) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

RAGE contributes to maintenance of pulmonary mechanics and structure.Quantification of mean chord length (A) were performed by stereological analysis of alveolar parenchyma; n ≥ 4 per group. (B) Representative histology (hematoxylin and eosin staining) of ten months old WT and RAGE-/- mice; scale bars: 200μm. Respiratory system compliance (C) and respiratory system elastance (D) were determined in two, four and ten months old WT and RAGE-/- mice using invasive pulmonary function measurements; n ≥ 7 per group. (E) Concentration of serum protein albumin in BALF of two months old mice; n = 10. Data are shown as mean ± SEM. *p < 0.05 and **p < 0.01. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0180092), licensed under a CC-BY license. Not internally tested by R&D Systems.

RAGE is expressed on alveolar epithelial cells and alveolar macrophages.(A) Immunostaining for RAGE protein in WT and RAGE-/- lung tissue. (B) Isolated alveolar epithelial cells (AEC) (n = 7 per group) and alveolar macrophages (AM) (n = 4 per group) were analyzed for RAGE mRNA expression using qRT-PCR. Data are shown as mean ± SEM. Scale bar: 100μm. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0180092), licensed under a CC-BY license. Not internally tested by R&D Systems.

Metformin activates AMPK and inhibits AGEs-induced RAGE expression and NF kappa B activation. (a) BMDMs were divided into 4 groups: control, AGEs, MET, and AGEs + MET group. In AGEs group, cells were cultured with AGEs at 200 mg/L for 24 h; in MET group, cells were cultured with metformin at 2.0 μM for 24 h; in AGEs + MET group, cells were pretreated with metformin for 60 min and then cultured with AGEs at 200 mg/L for 24 h; in control group, cells were cultured with BSA at 200 mg/L for 24 h. Western blot analysis was performed to measure protein levels of RAGE and phosphorylated AMPK (p-AMPK). Tubulin was used as internal control. (b) BMDMs were pretreated with or without metformin (2.0 μM) for 60 min before AGEs (200 mg/L) stimulation for different time intervals (0, 30, 60, and 180 min). Protein levels of NF kappa B-p65 (p65) and phosphorylated NF kappa B-p65 (p-p65) were measured by western blot. Tubulin was used as internal control. Bar graphs represent the results (mean ± SD) of three independent experiments. One-way ANOVA was applied and all the overall ANOVA was significant. #p > 0.05; ∗p < 0.05; ∗∗p < 0.01; and ∗∗∗p < 0.001 when compared between selected groups. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27761470), licensed under a CC-BY license. Not internally tested by R&D Systems.

A RAGE mRNA expression was elevated in lungs from RAGE TG mice compared to WT controls in the absence of SHS exposure (p = 0.02). RAGE mRNA was significantly elevated in WT (p = 0.02) and RAGE TG mice (p = 0.03) following exposure to SHS compared to room air (RA) controls. Expression of RAGE transcripts were not detected in RAGE KO mice regardless of exposure. The mRNA was normalized to beta -actin (n = 6 mice per group) and representative data are shown. B Analysis of RAGE protein demonstrated that RAGE TG animals expressed significantly more RAGE protein compared to controls (p = 0.02). SHS exposure significantly increased RAGE protein expression in WT (p = 0.01) and RAGE TG mice (p = 0.04) compared to RA controls while RAGE KO animals had no expression. Blots were densitometrically normalized to beta -actin and representative blots were cropped and presented Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35473605), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1179U-100UG
Alexa Fluor 405	AF1179V-100UG
Alexa Fluor 488	AF1179G-100UG
Alexa Fluor 532	AF1179X-100UG
Alexa Fluor 594	AF1179T-100UG
Alexa Fluor 647	AF1179R-100UG
Alexa Fluor 700	AF1179N-100UG
Alexa Fluor 750	AF1179S-100UG
Alexa Fluor Plus 405	AF1179AFP405-100UG
Alexa Fluor Plus 488	AF1179AFP488-100UG
Alexa Fluor Plus 555	AF1179AFP555-100UG
Alexa Fluor Plus 594	AF1179AFP594-100UG
Alexa Fluor Plus 647	AF1179AFP647-100UG
Alexa Fluor Plus 680	AF1179AFP680-100UG
Allophycocyanin	AF1179A
Biotin	BAF1179
CoraFluor 1	AF1179CL1
DyLight 350	AF1179D
DyLight 405	AF1179E
DyLight 488	AF1179K
DyLight 550	AF1179L
DyLight 594	AF1179M
DyLight 650	AF1179W
DyLight 680	AF1179Y
DyLight 755	AF1179Z
FITC	AF1179F
HRP	AF1179H
Janelia Fluor 525	AF1179JF525
Janelia Fluor 549	AF1179I
Janelia Fluor 646	AF1179J
Janelia Fluor 669	AF1179JF669
PE	AF1179P
PerCP	AF1179C
mFluor Violet 450 SE	AF1179MFV450
mFluor Violet 500 SE	AF1179MFV500
mFluor Violet 610 SE	AF1179MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1179-SP

compare

(select up to 3 products from this page to compare)

MAB11451

AGER Antibody (176902) [Unconjugated]

(34 Publications)

100 μg

MAB11451

HAF007). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human RAGE Monoclonal Antibody (Catalog # MAB11451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human RAGE Monoclonal Antibody (Catalog # MAB11451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

The effects of blocking TLR4 and RAGE on S100A8, S100A9 and S100A8/A9 stimulation of HUVECs.HUVECs were stimulated with S100A8 (2.0 µg/mL) (A, D), S100A9 (2.0 µg/mL) (B, E) and S100A8/A9 (2.0 µg/mL) (C, F) for 120 min with or without 60 min pre-incubation with specific blockers (TAK242 (5 µM) for TLR4 and anti-human RAGE antibody (10 µg/mL) for RAGE). Then the TER was measured (A, B and C). All data are presented as mean ± s.d. of four independent experiments. Phosphorylation of p38 (p-p38) and ERK1/2 (p-ERK1/2) were also assessed by Western blotting (D, E and F). The ratio of immunointensity between the phosphorylation of p38 and ERK1/2 (p-p38 and p-ERK) and total p38 and ERK1/2 (p38 and ERK) were calculated (D, E and F). The results are expressed in mean ± s.d. from three independent experiments. **P<0.01 vs. Control; #P<0.05 vs. S100A8/S100A9; ♣P<0.05 vs. S100A8/S100A9+TAK242+anti-RAGE. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24595267), licensed under a CC-BY license. Not internally tested by R&D Systems.

1145-RG) was serially diluted and captured by Mouse Anti-Human RAGE/AGER Monoclonal Antibody (Catalog # MAB11451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse/Rat RAGE/AGER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

1145-RG) was serially diluted and captured by Mouse Anti-Human RAGE/AGER Monoclonal Antibody (Catalog # MAB11451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse/Rat RAGE/AGER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB11451U-100UG
Alexa Fluor 405	FAB11451V-100UG
Alexa Fluor 488	FAB11451G-100UG
Alexa Fluor 532	FAB11451X-100UG
Alexa Fluor 594	FAB11451T-100UG
Alexa Fluor 647	FAB11451R-100UG
Alexa Fluor 700	FAB11451N-100UG
Alexa Fluor 750	FAB11451S-100UG
Alexa Fluor Plus 405	FAB11451AFP405-100UG
Alexa Fluor Plus 488	FAB11451AFP488-100UG
Alexa Fluor Plus 555	FAB11451AFP555-100UG
Alexa Fluor Plus 594	FAB11451AFP594-100UG
Alexa Fluor Plus 647	FAB11451AFP647-100UG
Alexa Fluor Plus 680	FAB11451AFP680-100UG
Allophycocyanin	FAB11451A
Biotin	FAB11451B
CoraFluor 1	FAB11451CL1
DyLight 350	FAB11451D
DyLight 405	FAB11451E
DyLight 488	FAB11451K
DyLight 550	FAB11451L
DyLight 594	FAB11451M
DyLight 650	FAB11451W
DyLight 680	FAB11451Y
DyLight 755	FAB11451Z
FITC	FAB11451F
HRP	FAB11451H
Janelia Fluor 525	FAB11451JF525
Janelia Fluor 549	FAB11451I
Janelia Fluor 646	FAB11451J
Janelia Fluor 669	FAB11451JF669
PE	FAB11451P
PerCP	FAB11451C
mFluor Violet 450 SE	FAB11451MFV450
mFluor Violet 500 SE	FAB11451MFV500
mFluor Violet 610 SE	FAB11451MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11451-SP
Carrier Free	MAB11451-500

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DY1145

Human RAGE DuoSet ELISA, 15 Plate

(28 Publications)

1 Kit (for 15 Plates)

DY1145

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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1145-RG

Recombinant Human RAGE Fc Chimera Protein, CF

(20 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MRG00

Mouse RAGE Quantikine ELISA Kit

(15 Publications)

1 Kit

MRG00

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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MAB1145

AGER Antibody (176907) [Unconjugated]

(7 Publications)

500 μg

MAB1145

HAF007). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF007). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human RAGE Monoclonal Antibody (Catalog # MAB1145) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

RAGE was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human RAGE Monoclonal Antibody (Catalog # MAB1145) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Validation of RAGE-overexpressing BC cells. A Kaplan–Meier plot showing the association of RAGE mRNA expression with the progression free interval (PFI) of the TCGA ER-positive BC patients. Samples were divided into RAGE high and low groups using the optimum cut-off. B-C Morphological appearance of wild type (MCF7/wt and T47D/wt) and RAGE-overexpressing (MCF7/RAGE and T47D/RAGE) cells in phase-contrast microscopy; scale bar: 250 μm. Enlarged details are shown in the separate boxes. Flow cytometric histograms in RAGE-overexpressing compared with wild type MCF7 (D) and T47D (E) cells. FITC, fluorescein isothiocyanate. Side panels show the percentage of RAGE-positive cells. F-G Immunoblots of RAGE in wild type (MCF7/wt and T47D/wt) and RAGE-overexpressing (MCF7/RAGE and T47D/RAGE) cells. Side panels show densitometric analysis of the blots normalized to beta -actin, which was used as a loading control. Values represent the mean ± SD of three independent experiments performed in triplicate. H-I Evaluation of RAGE protein expression (green signal) by immunofluorescence experiment in wild type (MCF7/wt and T47D/wt) and RAGE-overexpressing (MCF7/RAGE and T47D/RAGE) cells; nuclei were stained by DAPI (blue signal). The images shown represent 10 random fields from three independent experiments. Scale bar: 25 μm. Side panels represent corrected total cell fluorescence (CTCF), which was calculated on at least 10 pictures from each sample. (*) indicates p < 0.05; (**) indicates p < 0.01; (**) indicates p < 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37434266), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1145U-100UG
Alexa Fluor 405	FAB1145V-100UG
Alexa Fluor 488	FAB1145G-100UG
Alexa Fluor 532	FAB1145X-100UG
Alexa Fluor 594	FAB1145T-100UG
Alexa Fluor 647	FAB1145R-100UG
Alexa Fluor 700	FAB1145N-100UG
Alexa Fluor 750	FAB1145S-100UG
Alexa Fluor Plus 405	FAB1145AFP405-100UG
Alexa Fluor Plus 488	FAB1145AFP488-100UG
Alexa Fluor Plus 555	FAB1145AFP555-100UG
Alexa Fluor Plus 594	FAB1145AFP594-100UG
Alexa Fluor Plus 647	FAB1145AFP647-100UG
Alexa Fluor Plus 680	FAB1145AFP680-100UG
Allophycocyanin	FAB1145A
Biotin	FAB1145B
CoraFluor 1	FAB1145CL1
DyLight 350	FAB1145D
DyLight 405	FAB1145E
DyLight 488	FAB1145K
DyLight 550	FAB1145L
DyLight 594	FAB1145M
DyLight 650	FAB1145W
DyLight 680	FAB1145Y
DyLight 755	FAB1145Z
FITC	FAB1145F
HRP	FAB1145H
Janelia Fluor 525	FAB1145JF525
Janelia Fluor 549	FAB1145I
Janelia Fluor 646	FAB1145J
Janelia Fluor 669	FAB1145JF669
PE	FAB1145P
PerCP	FAB1145C
mFluor Violet 450 SE	FAB1145MFV450
mFluor Violet 500 SE	FAB1145MFV500
mFluor Violet 610 SE	FAB1145MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1145-SP

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NBP2-67095

AGER Antibody (JF0975)

(1 Review)

100 ul

NBP2-67095

Immunohistochemistry-Paraffin: AGER Antibody (JF0975) [NBP2-67095] - Mouse lung cancer tissue stained with AGER antibody. IHC-P image submitted by a verified customer review.

Flow Cytometry: AGER Antibody (JF0975) [NBP2-67095] - Analysis of Jurkat cells with RAGE antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: AGER Antibody (JF0975) [NBP2-67095] - Staining RAGE in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: AGER Antibody (JF0975) [NBP2-67095] - Analysis of paraffin-embedded mouse lung tissue using anti-RAGE antibody. Counter stained with hematoxylin.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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AF1616

AGER Antibody

(4 Publications)

100 μg

AF1616

The effect of pitavastatin on AGEs-induced autophagy. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29089470), licensed under a CC-BY license. Not internally tested by R&D Systems.

1616-RG) was serially diluted and captured by Goat Anti-Rat RAGE/AGER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1616) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat RAGE/AGER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1616) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Rat RAGE/AGER Fc Chimera (Catalog # <a class=

1616-RG) was serially diluted and captured by Goat Anti-Rat RAGE/AGER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1616) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat RAGE/AGER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1616) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1616-RG) was serially diluted and captured by Goat Anti-Rat RAGE/AGER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1616) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat RAGE/AGER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1616) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

1616-RG) was serially diluted and captured by Goat Anti-Rat RAGE/AGER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1616) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat RAGE/AGER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1616) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1616U-100UG
Alexa Fluor 405	AF1616V-100UG
Alexa Fluor 488	AF1616G-100UG
Alexa Fluor 532	AF1616X-100UG
Alexa Fluor 594	AF1616T-100UG
Alexa Fluor 647	AF1616R-100UG
Alexa Fluor 700	AF1616N-100UG
Alexa Fluor 750	AF1616S-100UG
Alexa Fluor Plus 405	AF1616AFP405-100UG
Alexa Fluor Plus 488	AF1616AFP488-100UG
Alexa Fluor Plus 555	AF1616AFP555-100UG
Alexa Fluor Plus 594	AF1616AFP594-100UG
Alexa Fluor Plus 647	AF1616AFP647-100UG
Alexa Fluor Plus 680	AF1616AFP680-100UG
Allophycocyanin	AF1616A
Biotin	BAF1616
CoraFluor 1	AF1616CL1
DyLight 350	AF1616D
DyLight 405	AF1616E
DyLight 488	AF1616K
DyLight 550	AF1616L
DyLight 594	AF1616M
DyLight 650	AF1616W
DyLight 680	AF1616Y
DyLight 755	AF1616Z
FITC	AF1616F
HRP	AF1616H
Janelia Fluor 525	AF1616JF525
Janelia Fluor 549	AF1616I
Janelia Fluor 646	AF1616J
Janelia Fluor 669	AF1616JF669
PE	AF1616P
PerCP	AF1616C
mFluor Violet 450 SE	AF1616MFV450
mFluor Violet 500 SE	AF1616MFV500
mFluor Violet 610 SE	AF1616MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1616-SP

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DY1179

Mouse RAGE DuoSet ELISA, 15 Plate

(9 Publications)

1 Kit (for 15 Plates)

DY1179

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Preservative

Protein Or Peptide

Has Sample Size

Promotional Item

Species Glossary

AGER Products By Gene Name

Human RAGE Quantikine ELISA Kit

AGER Antibody (175410) [Unconjugated]

AGER Antibody [Unconjugated]

AGER Antibody [Unconjugated]

AGER Antibody (176902) [Unconjugated]

Human RAGE DuoSet ELISA, 15 Plate

Recombinant Human RAGE Fc Chimera Protein, CF

Mouse RAGE Quantikine ELISA Kit

AGER Antibody (176907) [Unconjugated]

AGER Antibody (JF0975)

AGER Antibody

Mouse RAGE DuoSet ELISA, 15 Plate